24 Citations (Scopus)

Abstract

In this paper we report on the fabrication and performance of a portable and low cost optoelectronic platform integrating a double color tuned light emitting diode as light source, an amorphous/nanocrystalline silicon photodetector with a flat spectral response in the wavelength range from 520nm to 630nm and integrated electronic for signal acquisition and conditioning constituted by current to voltage converter, a filter and an amplification stage, followed by an analog to digital converter, with appropriate software for full automation to minimize human error. Incorporation of the double color tuned light emitting diode provides for a simple yet innovative solution to signal acquisition independently from the light intensity and/or solution concentration, while considerably decreasing production costs. Detection based on Au-nanoprobes constitutes the biorecognition step and allowed identification of specific sequences ofMycobacterium tuberculosiscomplex, namelyMycobacterium bovisandM. tuberculosisin biological samples.
Original languageUnknown
Pages (from-to)2012-2017
JournalBiosensors & Bioelectronics
Volume26
Issue number5
DOIs
Publication statusPublished - 1 Jan 2011

Cite this

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title = "Portable optoelectronic biosensing platform for identification of mycobacteria from the Mycobacterium tuberculosis complex",
abstract = "In this paper we report on the fabrication and performance of a portable and low cost optoelectronic platform integrating a double color tuned light emitting diode as light source, an amorphous/nanocrystalline silicon photodetector with a flat spectral response in the wavelength range from 520nm to 630nm and integrated electronic for signal acquisition and conditioning constituted by current to voltage converter, a filter and an amplification stage, followed by an analog to digital converter, with appropriate software for full automation to minimize human error. Incorporation of the double color tuned light emitting diode provides for a simple yet innovative solution to signal acquisition independently from the light intensity and/or solution concentration, while considerably decreasing production costs. Detection based on Au-nanoprobes constitutes the biorecognition step and allowed identification of specific sequences ofMycobacterium tuberculosiscomplex, namelyMycobacterium bovisandM. tuberculosisin biological samples.",
keywords = "Amorphous thin film silicon color sensor, Mycobacterium tuberculosis, Tuberculosis, DNA, Optoelectronic platform, Gold-nanoparticles",
author = "Fortunato, {Elvira Maria Correia} and Martins, {Rodrigo Ferr{\~a}o de Paiva} and Baptista, {Pedro Miguel Ribeiro Viana}",
year = "2011",
month = "1",
day = "1",
doi = "10.1016/j.bios.2010.08.078",
language = "Unknown",
volume = "26",
pages = "2012--2017",
journal = "Biosensors & Bioelectronics",
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publisher = "Elsevier Science B.V., Amsterdam.",
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TY - JOUR

T1 - Portable optoelectronic biosensing platform for identification of mycobacteria from the Mycobacterium tuberculosis complex

AU - Fortunato, Elvira Maria Correia

AU - Martins, Rodrigo Ferrão de Paiva

AU - Baptista, Pedro Miguel Ribeiro Viana

PY - 2011/1/1

Y1 - 2011/1/1

N2 - In this paper we report on the fabrication and performance of a portable and low cost optoelectronic platform integrating a double color tuned light emitting diode as light source, an amorphous/nanocrystalline silicon photodetector with a flat spectral response in the wavelength range from 520nm to 630nm and integrated electronic for signal acquisition and conditioning constituted by current to voltage converter, a filter and an amplification stage, followed by an analog to digital converter, with appropriate software for full automation to minimize human error. Incorporation of the double color tuned light emitting diode provides for a simple yet innovative solution to signal acquisition independently from the light intensity and/or solution concentration, while considerably decreasing production costs. Detection based on Au-nanoprobes constitutes the biorecognition step and allowed identification of specific sequences ofMycobacterium tuberculosiscomplex, namelyMycobacterium bovisandM. tuberculosisin biological samples.

AB - In this paper we report on the fabrication and performance of a portable and low cost optoelectronic platform integrating a double color tuned light emitting diode as light source, an amorphous/nanocrystalline silicon photodetector with a flat spectral response in the wavelength range from 520nm to 630nm and integrated electronic for signal acquisition and conditioning constituted by current to voltage converter, a filter and an amplification stage, followed by an analog to digital converter, with appropriate software for full automation to minimize human error. Incorporation of the double color tuned light emitting diode provides for a simple yet innovative solution to signal acquisition independently from the light intensity and/or solution concentration, while considerably decreasing production costs. Detection based on Au-nanoprobes constitutes the biorecognition step and allowed identification of specific sequences ofMycobacterium tuberculosiscomplex, namelyMycobacterium bovisandM. tuberculosisin biological samples.

KW - Amorphous thin film silicon color sensor

KW - Mycobacterium tuberculosis

KW - Tuberculosis

KW - DNA

KW - Optoelectronic platform

KW - Gold-nanoparticles

U2 - 10.1016/j.bios.2010.08.078

DO - 10.1016/j.bios.2010.08.078

M3 - Article

VL - 26

SP - 2012

EP - 2017

JO - Biosensors & Bioelectronics

JF - Biosensors & Bioelectronics

SN - 0956-5663

IS - 5

ER -