12 Citations (Scopus)

Abstract

The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are 'smart' biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.
Original languageUnknown
Pages (from-to)54872-54878
JournalRSC ADVANCES
Volume4
Issue number97
DOIs
Publication statusPublished - 1 Jan 2014

Cite this

@article{566d71eb0e3a48a1a642fee0eba9cc6b,
title = "Polyurea dendrimer for efficient cytosolic siRNA delivery",
abstract = "The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are 'smart' biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.",
keywords = "THERAPEUTICS, CANCER, RNA INTERFERENCE, PROGRESS, PAMAM DENDRIMERS, RELAXATION, CONFORMATION, DESIGN, NMR, MOLECULES",
author = "Cidade, {Maria Teresa Varanda} and Pedro Baptista",
year = "2014",
month = "1",
day = "1",
doi = "10.1039/c4ra09603g",
language = "Unknown",
volume = "4",
pages = "54872--54878",
journal = "RSC ADVANCES",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
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}

Polyurea dendrimer for efficient cytosolic siRNA delivery. / Cidade, Maria Teresa Varanda; Baptista, Pedro.

In: RSC ADVANCES, Vol. 4, No. 97, 01.01.2014, p. 54872-54878.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Polyurea dendrimer for efficient cytosolic siRNA delivery

AU - Cidade, Maria Teresa Varanda

AU - Baptista, Pedro

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are 'smart' biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.

AB - The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are 'smart' biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.

KW - THERAPEUTICS

KW - CANCER

KW - RNA INTERFERENCE

KW - PROGRESS

KW - PAMAM DENDRIMERS

KW - RELAXATION

KW - CONFORMATION

KW - DESIGN

KW - NMR

KW - MOLECULES

U2 - 10.1039/c4ra09603g

DO - 10.1039/c4ra09603g

M3 - Article

VL - 4

SP - 54872

EP - 54878

JO - RSC ADVANCES

JF - RSC ADVANCES

SN - 2046-2069

IS - 97

ER -