TY - JOUR
T1 - Polyphasic taxonomy of the basidiomycetous yeast genus Rhodosporidium
T2 - R. azoricum sp. nov.
AU - Gadanho, Mário
AU - Sampaio, José Paulo Nunes de Sousa
AU - Spencer-Martins, Isabel
N1 - Sem pdf.
PY - 2001
Y1 - 2001
N2 - This report presents the description of a new heterothallic Rhodosporidium species, R. azoricum sp. nov. The new species is based on two strains previously identified as Rhodotorula glutinis, which were isolated from soil in São Miguel island, Azores, Portugal. Evidence that the two strains were conspecific and distinct from Rhodotorula glutinis was obtained in DNA fingerprinting experiments using the microsatellite-primed PCR approach (MSP-PCR) and the primers M13 and (GTG)5. In order to determine the phylogenetic position of the new species, the nucleotide sequence of the D1/D2 region of the 26S rDNA was analysed and Rhodosporidium azoricum was found to belong to a cluster including R. fluviale, R. lusitaniae, Sporidiobolus microsporus, and S. ruineniae. The life cycle of R. azoricum was investigated and comparisons integrating physiological, morphological, and molecular data were made with related species.
AB - This report presents the description of a new heterothallic Rhodosporidium species, R. azoricum sp. nov. The new species is based on two strains previously identified as Rhodotorula glutinis, which were isolated from soil in São Miguel island, Azores, Portugal. Evidence that the two strains were conspecific and distinct from Rhodotorula glutinis was obtained in DNA fingerprinting experiments using the microsatellite-primed PCR approach (MSP-PCR) and the primers M13 and (GTG)5. In order to determine the phylogenetic position of the new species, the nucleotide sequence of the D1/D2 region of the 26S rDNA was analysed and Rhodosporidium azoricum was found to belong to a cluster including R. fluviale, R. lusitaniae, Sporidiobolus microsporus, and S. ruineniae. The life cycle of R. azoricum was investigated and comparisons integrating physiological, morphological, and molecular data were made with related species.
KW - Basidiomycetous yeasts
KW - Microsatellite-primed PCR
KW - Rhodosporidium azoricum sp. nov.
KW - Rhodotorula glutinis
UR - http://www.scopus.com/inward/record.url?scp=0035061196&partnerID=8YFLogxK
U2 - 10.1139/cjm-47-3-213
DO - 10.1139/cjm-47-3-213
M3 - Article
C2 - 11315112
SN - 0008-4166
VL - 47
SP - 213
EP - 221
JO - Canadian Journal Of Microbiology
JF - Canadian Journal Of Microbiology
IS - 3
ER -