Polymorphism in the IL-1β promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax

Marcela Petrolini Capobianco, Gustavo Capatti Cassiano, Luciane Moreno Storti-Melo, Tamirys Simão Pimenta, Ana Paula Drummond Rodrigues, José Eduardo Gomes Arruda, Marcia Ribeiro Pinto, Andrea Regina De Souza Baptista, Lilian Rose Pratt-Riccio, Cláudia Regina Bonini-Domingos, Joseli De Oliveira-Ferreira, Ricardo Luiz Dantas Machado

Research output: Contribution to journalArticlepeer-review


Background: It is well established that infection by Plasmodium vivax is a result of host-parasite interactions. In the present study, association with the IL1/IL2 cytokine profiles, anticircumsporozoite protein antibody levels and parasitic loads was evaluated in individuals naturally infected with P. vivax in an endemic area of the Brazilian Amazon. Methods: Molecular diagnosis of P. vivax and variants was performed using the PCR-RFLP method and IL1B - 511C>T, IL2 -330T>G and IL2+114T>G polymorphisms were identified using PCR-RFLP and allele-specific PCR. IL-1β and IL-2 cytokine levels were detected by flow cytometry and circumsporozoite protein (CSP) antibodies were measured by ELISA. Results: Three variants of P. vivax CSP were identified and VK247 was found to be the most frequent. However, the prevalence and magnitude of IgG antibodies were higher for the VK210 variant. Furthermore, the antibody response to the CSP variants was not associated with the presence of the variant in the infection. Significant differences were observed between the single nucleotide polymorphism (SNP) -511T>C in the IL1B gene and levels of antibodies to the VK247 and P. vivax-like variants, but there were no associations between SNPs in IL1 and IL2 genes and their plasma products. Conclusions: Individuals with the rs16944 CC genotype in the IL1β gene have higher antibody levels to the CSP of P. vivax of VK247 and P. vivax-like variants.

Original languageEnglish
Pages (from-to)858-865
Number of pages8
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Issue number11
Publication statusPublished - 6 Nov 2020


  • Antibodies
  • Circumsporozoite protein
  • IL-1
  • IL-2
  • Plasmodium vivax
  • Polymorphisms


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