TY - JOUR
T1 - Polymorphism in the IL-1β promoter is associated with IgG antibody response to circumsporozoite protein repeats of Plasmodium vivax
AU - Capobianco, Marcela Petrolini
AU - Cassiano, Gustavo Capatti
AU - Storti-Melo, Luciane Moreno
AU - Pimenta, Tamirys Simão
AU - Rodrigues, Ana Paula Drummond
AU - Arruda, José Eduardo Gomes
AU - Pinto, Marcia Ribeiro
AU - De Souza Baptista, Andrea Regina
AU - Pratt-Riccio, Lilian Rose
AU - Bonini-Domingos, Cláudia Regina
AU - De Oliveira-Ferreira, Joseli
AU - Machado, Ricardo Luiz Dantas
PY - 2020/11/6
Y1 - 2020/11/6
N2 - Background: It is well established that infection by Plasmodium vivax is a result of host-parasite interactions. In the present study, association with the IL1/IL2 cytokine profiles, anticircumsporozoite protein antibody levels and parasitic loads was evaluated in individuals naturally infected with P. vivax in an endemic area of the Brazilian Amazon. Methods: Molecular diagnosis of P. vivax and variants was performed using the PCR-RFLP method and IL1B - 511C>T, IL2 -330T>G and IL2+114T>G polymorphisms were identified using PCR-RFLP and allele-specific PCR. IL-1β and IL-2 cytokine levels were detected by flow cytometry and circumsporozoite protein (CSP) antibodies were measured by ELISA. Results: Three variants of P. vivax CSP were identified and VK247 was found to be the most frequent. However, the prevalence and magnitude of IgG antibodies were higher for the VK210 variant. Furthermore, the antibody response to the CSP variants was not associated with the presence of the variant in the infection. Significant differences were observed between the single nucleotide polymorphism (SNP) -511T>C in the IL1B gene and levels of antibodies to the VK247 and P. vivax-like variants, but there were no associations between SNPs in IL1 and IL2 genes and their plasma products. Conclusions: Individuals with the rs16944 CC genotype in the IL1β gene have higher antibody levels to the CSP of P. vivax of VK247 and P. vivax-like variants.
AB - Background: It is well established that infection by Plasmodium vivax is a result of host-parasite interactions. In the present study, association with the IL1/IL2 cytokine profiles, anticircumsporozoite protein antibody levels and parasitic loads was evaluated in individuals naturally infected with P. vivax in an endemic area of the Brazilian Amazon. Methods: Molecular diagnosis of P. vivax and variants was performed using the PCR-RFLP method and IL1B - 511C>T, IL2 -330T>G and IL2+114T>G polymorphisms were identified using PCR-RFLP and allele-specific PCR. IL-1β and IL-2 cytokine levels were detected by flow cytometry and circumsporozoite protein (CSP) antibodies were measured by ELISA. Results: Three variants of P. vivax CSP were identified and VK247 was found to be the most frequent. However, the prevalence and magnitude of IgG antibodies were higher for the VK210 variant. Furthermore, the antibody response to the CSP variants was not associated with the presence of the variant in the infection. Significant differences were observed between the single nucleotide polymorphism (SNP) -511T>C in the IL1B gene and levels of antibodies to the VK247 and P. vivax-like variants, but there were no associations between SNPs in IL1 and IL2 genes and their plasma products. Conclusions: Individuals with the rs16944 CC genotype in the IL1β gene have higher antibody levels to the CSP of P. vivax of VK247 and P. vivax-like variants.
KW - Antibodies
KW - Circumsporozoite protein
KW - IL-1
KW - IL-2
KW - Plasmodium vivax
KW - Polymorphisms
UR - http://www.scopus.com/inward/record.url?scp=85095862543&partnerID=8YFLogxK
UR - https://academic.oup.com/trstmh/article/114/11/858/5885183
U2 - 10.1093/trstmh/traa055
DO - 10.1093/trstmh/traa055
M3 - Article
C2 - 32766886
AN - SCOPUS:85095862543
VL - 114
SP - 858
EP - 865
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
SN - 0035-9203
IS - 11
ER -