Polymeric scaffolds for enhanced stability of melanin incorporated in liposomes

Marli L. Moraes, Paulo J. Gomes, Paulo A. Ribeiro, Pedro Vieira, Adilson A. Freitas, Ralf Köhler, Osvaldo N. Oliveira Jr., Maria Raposo

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


The use of melanin in bioinspired applications is mostly limited by its poor stability in solid films. This problem has been addressed here by incorporating melanin into dipalmitoyl phosphatidyl glycerol (DPPG) liposomes, which were then immobilized onto a solid substrate as an LbL film. Results from steady-state and time-resolved fluorescence indicated an increased stability for melanin incorporated into DPPG liposomes. If not protected by liposomes, melanin looses completely its fluorescence properties in LbL films. The thickness of the liposome-melanin layer obtained from neutron reflectivity data was 4.1 +/- 0.2 nm, consistent with the value estimated for the phospholipid bilayer of the liposomes, an evidence of the collapse of most liposomes. On the other hand, the final roughness indicated that some of the liposomes had their structure preserved. In summary, liposomes were proven excellent for encapsulation, thus providing a suitable environment, closer to the physiological conditions without using organic solvents or high pHs. (C) 2010 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)268-274
Number of pages7
JournalJournal of Colloid and Interface Science
Issue number1
Publication statusPublished - 1 Oct 2010


  • Dipalmitoyl phosphatidyl glycerol (DPPG)
  • Fluorescence
  • Layer-by-layer films
  • Liposome
  • Melanin
  • Neutron reflectivity


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