TY - JOUR
T1 - Polyhydroxyalkanoates from industrial cheese whey
T2 - Production and characterization of polymers with differing hydroxyvalerate content
AU - Carvalheira, Mónica
AU - Hilliou, Loic
AU - Oliveira, Catarina S. S.
AU - Guarda, Eliana C.
AU - Reis, Maria A.M.
N1 - Funding Information:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT#
and project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy–i4HB.
Funding Information:
The authors acknowledge financial support from the European project YPack (H2020-SFS-35-2017) and additional financial support from the FCT–Fundação para a Ciência e a Tecnologia, I.P. under the framework of Strategic Funding grant UID/CTM/50025/2020.
Funding Information:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT#
The authors acknowledge financial support from the European project YPack (H2020-SFS-35-2017) and additional financial support from the FCT?Funda??o para a Ci?ncia e a Tecnologia, I.P. under the framework of Strategic Funding grant UID/CTM/50025/2020. Funding, of the Research Unit on Applied Molecular Biosciences?UCIBIO and project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy?i4HB.
Publisher Copyright:
© 2022 The Authors
PY - 2022/1
Y1 - 2022/1
N2 - The composition of polyhydroxyalkanoate (PHA) monomers affects the properties and final applications of PHA polymers. This study focused on the feasibility of producing tailored PHA differing in hydroxyvalerate (HV) content, through manipulation of the acidogenic fermented stream composition, and on the characterization of PHA properties to determine the best composition for melt processing. Cheese whey was used as a feedstock, and changes in the organic loading rate during acidogenic fermentation led to the production of a fermented stream with an HV precursor content of 9–33 wt%. In the PHA production assays, a PHA content of 50 ± 11 wt%. (VSS basis) and yield of 0.76 ± 0.14 gCODPHA.gCODFP-1 were obtained. Fermented stream supplementation with HV precursors during the PHA accumulation assays indicated the feasibility of producing tailored PHA with differing HV content without a need for the selection of new cultures. The thermal properties of PHA were found to be controlled by the HV content, and PHA with approximately 30 wt% HV had the lowest melting temperature. These results demonstrated the robustness of the process at pilot scale, thus supporting full-scale applications in tailored PHA production.
AB - The composition of polyhydroxyalkanoate (PHA) monomers affects the properties and final applications of PHA polymers. This study focused on the feasibility of producing tailored PHA differing in hydroxyvalerate (HV) content, through manipulation of the acidogenic fermented stream composition, and on the characterization of PHA properties to determine the best composition for melt processing. Cheese whey was used as a feedstock, and changes in the organic loading rate during acidogenic fermentation led to the production of a fermented stream with an HV precursor content of 9–33 wt%. In the PHA production assays, a PHA content of 50 ± 11 wt%. (VSS basis) and yield of 0.76 ± 0.14 gCODPHA.gCODFP-1 were obtained. Fermented stream supplementation with HV precursors during the PHA accumulation assays indicated the feasibility of producing tailored PHA with differing HV content without a need for the selection of new cultures. The thermal properties of PHA were found to be controlled by the HV content, and PHA with approximately 30 wt% HV had the lowest melting temperature. These results demonstrated the robustness of the process at pilot scale, thus supporting full-scale applications in tailored PHA production.
KW - Biodegradable biopolymers
KW - Cheese whey valorization
KW - Hydroxyvalerate content
KW - Pilot Scale
KW - Thermal properties
UR - http://www.scopus.com/inward/record.url?scp=85127116697&partnerID=8YFLogxK
U2 - 10.1016/j.crbiot.2022.03.004
DO - 10.1016/j.crbiot.2022.03.004
M3 - Article
AN - SCOPUS:85127116697
SN - 2590-2628
VL - 4
SP - 211
EP - 220
JO - Current Research in Biotechnology
JF - Current Research in Biotechnology
ER -