Plasmodium yoelii: The effect of second blood meal and anti-sporozoite antibodies on development and gene expression in the mosquito vector, Anopheles stephensi

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Abstract

The sporogonic development of the malaria parasite takes place in the mosquito and a wide range of factors modulates it. Among those, the contents of the blood meal can influence the parasite development directly or indirectly through the mosquito response to the infection. We have studied the effect of a second blood meal in previously infected mosquitoes and the effect of anti-sporozoite immune serum on parasite development and mosquito response to the infection. The prevalence and intensity of infection and gene expression of both Plasmodium yoelii and Anopheles stephensi was analyzed. We verified that a second blood meal and its immune status interfere with parasite development and with Plasmodium and mosquito gene expression.

Original languageEnglish
Pages (from-to)259-269
Number of pages11
JournalExperimental Parasitology
Volume115
Issue number3
DOIs
Publication statusPublished - 1 Mar 2007

Keywords

  • α-[P]dATP
  • 4-aminobenzoic acid
  • 5′-[α
  • P] triphosphate
  • Anopheles stephensi
  • Apicomplexa
  • circumsporozoite protein
  • CS
  • DDRT-PCR
  • deoxyadenosine
  • deoxyribonucleic acid
  • deoxyribonucleotide triphosphate
  • differential display reverse transcriptase PCR
  • dithiothreitol
  • DNA
  • dNTP
  • DTT
  • IFA
  • IFN-γ
  • IgG
  • IHMT
  • Immune serum
  • immunoglobulin G
  • indirect fluorescent antibody
  • Instituto de Higiene e Medicina Tropical
  • interferon γ
  • Malaria
  • MMLV-RT
  • Moloney murine leukemia virus reverse transcriptase
  • nitric oxide
  • NO
  • PABA
  • PCR
  • Plasmodium yoelii
  • polymerase chain reaction
  • QT-PCR
  • quantitative real time PCR analysis
  • restriction fragment length polymorphism
  • RFLP
  • ribonucleic acid
  • RNA
  • Sporogonic development
  • TGF-β1
  • TNF-α
  • transport RNA
  • tRNA
  • tumor necrosis factor α
  • Tumor necrosis factor β one

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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