TY - JOUR
T1 - Plasmodium yoelii
T2 - semiquantitative analyses of circumsporozoite protein gene expression during the sporogonic development of P. y. yoelii and P. y. nigeriensis in the mosquito vector Anopheles stephensi
AU - Franco, Ana S
AU - Silveira, Henrique
AU - do Rosário, Virgílio E
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Malaria infection in the mosquito vector can be modulated by the vertebrate host, mosquito factors, and interactions between different parasite populations. Modulation of parasite development can be assessed through the study of gene expression. The present report describes a specific, sensitive, and nonradioactive method that permits assessment of parasite load and quantification of circumsporozoite protein gene expression during the sporogonic stages of Plasmodium yoelii yoelii and P. y. nigeriensis. A decrease in parasite load was observed when comparing DNA of oocysts on day 7 postinfection with that of oocysts and sporozoites on day 19. On day 7, parasites (oocysts) showed a marked increase of circumsporozoite protein expression when compared with that (sporozoites and oocysts) on day 19. The method developed in this work can be a valuable tool to understand parasite interaction mechanisms that are involved in mosquito malaria infections.
AB - Malaria infection in the mosquito vector can be modulated by the vertebrate host, mosquito factors, and interactions between different parasite populations. Modulation of parasite development can be assessed through the study of gene expression. The present report describes a specific, sensitive, and nonradioactive method that permits assessment of parasite load and quantification of circumsporozoite protein gene expression during the sporogonic stages of Plasmodium yoelii yoelii and P. y. nigeriensis. A decrease in parasite load was observed when comparing DNA of oocysts on day 7 postinfection with that of oocysts and sporozoites on day 19. On day 7, parasites (oocysts) showed a marked increase of circumsporozoite protein expression when compared with that (sporozoites and oocysts) on day 19. The method developed in this work can be a valuable tool to understand parasite interaction mechanisms that are involved in mosquito malaria infections.
KW - Animals
KW - Anopheles/parasitology
KW - DNA Primers
KW - DNA Probes
KW - DNA, Complementary/analysis
KW - DNA, Protozoan/analysis
KW - Female
KW - Gene Expression Regulation, Developmental
KW - Insect Vectors/parasitology
KW - Mice
KW - Plasmodium yoelii/genetics
KW - Protozoan Proteins/biosynthesis
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Sensitivity and Specificity
KW - Tetrahydrofolate Dehydrogenase/genetics
UR - https://bioone.org/journals/journal-of-parasitology/volume-89/issue-2/0022-3395(2003)089%5b0255%3aPYSAOC%5d2.0.CO%3b2/PLASMODIUM-YOELII--SEMIQUANTITATIVE-ANALYSES-OF-CIRCUMSPOROZOITE-PROTEIN-GENE-EXPRESSION/10.1645/0022-3395(2003)089[0255:PYSAOC]2.0.CO;2.short
U2 - 10.1645/0022-3395(2003)089[0255:PYSAOC]2.0.CO;2
DO - 10.1645/0022-3395(2003)089[0255:PYSAOC]2.0.CO;2
M3 - Article
C2 - 12760638
VL - 89
SP - 255
EP - 260
JO - Journal Of Parasitology
JF - Journal Of Parasitology
SN - 0022-3395
IS - 2
ER -