Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene

Francesca Guaracyaba Garcia Chapadense; Ricardo Luiz Dantas Machado; Ana Maria Revorêdo Da Silva Ventura; André Luiz Lisboa Areas; Renato Beilner Machado; Giselle Maria Rachid Viana; Mariano Gustavo Zalis; Ana Maria Castro; Pedro Vitor Lemos Cravo

doi:10.1590/0037-8682-0225-2018

Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene

Francesca Guaracyaba Garcia Chapadense, Ricardo Luiz Dantas Machado, Ana Maria Revorêdo Da Silva Ventura, André Luiz Lisboa Areas, Renato Beilner Machado, Giselle Maria Rachid Viana, Mariano Gustavo Zalis, Ana Maria Castro, Pedro Vitor Lemos Cravo

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6 Citations (Scopus)

Abstract

INTRODUCTION:
Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance.
METHODS:
We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil.
RESULTS:
We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples.
CONCLUSIONS:
Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.

Original language	English
Article number	e20180225
Number of pages	4
Journal	Revista Da Sociedade Brasileira De Medicina Tropical
Volume	52
DOIs	https://doi.org/10.1590/0037-8682-0225-2018
Publication status	Published - 21 Feb 2019

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SDG 3 Good Health and Well-being

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10.1590/0037-8682-0225-2018

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Chapadense, F. G. G., Machado, R. L. D., Ventura, A. M. R. D. S., Areas, A. L. L., Machado, R. B., Viana, G. M. R., Zalis, M. G., Castro, A. M., & Cravo, P. V. L. (2019). Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene. Revista Da Sociedade Brasileira De Medicina Tropical, 52, Article e20180225. https://doi.org/10.1590/0037-8682-0225-2018

@article{3ec7c308000f4b7fb8c62e67c4a5083a,

title = "Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene",

abstract = "INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36\% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.",

author = "Chapadense, \{Francesca Guaracyaba Garcia\} and Machado, \{Ricardo Luiz Dantas\} and Ventura, \{Ana Maria Revor{\^e}do Da Silva\} and Areas, \{Andr{\'e} Luiz Lisboa\} and Machado, \{Renato Beilner\} and Viana, \{Giselle Maria Rachid\} and Zalis, \{Mariano Gustavo\} and Castro, \{Ana Maria\} and Cravo, \{Pedro Vitor Lemos\}",

note = "Funding Information: Financial support: This project received partial federal support from the Funding Information: Evandro Chagas Institute/Brazilian Ministry of Health. This research was also partially financed by FAPESP (S{\~a}o Paulo Research Foundation) through Grant 02/9546-1 and CNPq (National Council for Scientific and Technological Development) through Grant 302353/2003-8 awarded to RLDM. RLDM is also supported by a fellowship from CNPq. AA is supported by CAPES (Federal Agency for the Support and Improvement of Higher Education). PC is supported by a fellowship from FUNADESP (National Foundation for Development of Private Education). These financing agencies played no role in study design, data collection and analysis, decision to publish, or manuscript preparation. Publisher Copyright: {\textcopyright} 2019, Sociedade Brasileira de Medicina Tropical. All rights reserved.",

year = "2019",

month = feb,

day = "21",

doi = "10.1590/0037-8682-0225-2018",

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journal = "Revista Da Sociedade Brasileira De Medicina Tropical",

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Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene. / Chapadense, Francesca Guaracyaba Garcia; Machado, Ricardo Luiz Dantas; Ventura, Ana Maria Revorêdo Da Silva et al.
In: Revista Da Sociedade Brasileira De Medicina Tropical, Vol. 52, e20180225, 21.02.2019.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene

AU - Chapadense, Francesca Guaracyaba Garcia

AU - Machado, Ricardo Luiz Dantas

AU - Ventura, Ana Maria Revorêdo Da Silva

AU - Areas, André Luiz Lisboa

AU - Machado, Renato Beilner

AU - Viana, Giselle Maria Rachid

AU - Zalis, Mariano Gustavo

AU - Castro, Ana Maria

AU - Cravo, Pedro Vitor Lemos

N1 - Funding Information: Financial support: This project received partial federal support from the Funding Information: Evandro Chagas Institute/Brazilian Ministry of Health. This research was also partially financed by FAPESP (São Paulo Research Foundation) through Grant 02/9546-1 and CNPq (National Council for Scientific and Technological Development) through Grant 302353/2003-8 awarded to RLDM. RLDM is also supported by a fellowship from CNPq. AA is supported by CAPES (Federal Agency for the Support and Improvement of Higher Education). PC is supported by a fellowship from FUNADESP (National Foundation for Development of Private Education). These financing agencies played no role in study design, data collection and analysis, decision to publish, or manuscript preparation. Publisher Copyright: © 2019, Sociedade Brasileira de Medicina Tropical. All rights reserved.

PY - 2019/2/21

Y1 - 2019/2/21

N2 - INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.

AB - INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.

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U2 - 10.1590/0037-8682-0225-2018

DO - 10.1590/0037-8682-0225-2018

M3 - Article

C2 - 30810653

SN - 0037-8682

VL - 52

JO - Revista Da Sociedade Brasileira De Medicina Tropical

JF - Revista Da Sociedade Brasileira De Medicina Tropical

M1 - e20180225

ER -

Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene

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