Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene

Francesca Guaracyaba Garcia Chapadense, Ricardo Luiz Dantas Machado, Ana Maria Revorêdo Da Silva Ventura, André Luiz Lisboa Areas, Renato Beilner Machado, Giselle Maria Rachid Viana, Mariano Gustavo Zalis, Ana Maria Castro, Pedro Vitor Lemos Cravo

Research output: Contribution to journalArticle

Abstract

INTRODUCTION:
Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance.
METHODS:
We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil.
RESULTS:
We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples.
CONCLUSIONS:
Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.
Original languageEnglish
Article numbere20180225
Number of pages4
JournalRevista Da Sociedade Brasileira De Medicina Tropical
Volume52
DOIs
Publication statusPublished - 21 Feb 2019

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  • SDG 3 - Good Health and Well-Being

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    Chapadense, F. G. G., Machado, R. L. D., Ventura, A. M. R. D. S., Areas, A. L. L., Machado, R. B., Viana, G. M. R., Zalis, M. G., Castro, A. M., & Cravo, P. V. L. (2019). Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene. Revista Da Sociedade Brasileira De Medicina Tropical, 52, [e20180225]. https://doi.org/10.1590/0037-8682-0225-2018