Plasmodium chabaudi: Expression of active recombinant chabaupain-1 and localization studies in Anopheles sp.

Rubina L. Caldeira; Lídia M.D. Gonçalves; Tiago M. Martins; Henrique Silveira; Carlos Novo; Virgílio do Rosário; Ana Domingos

doi:10.1016/j.exppara.2009.03.003

Plasmodium chabaudi: Expression of active recombinant chabaupain-1 and localization studies in Anopheles sp.

Rubina L. Caldeira, Lídia M.D. Gonçalves, Tiago M. Martins, Henrique Silveira, Carlos Novo, Virgílio do Rosário, Ana Domingos

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction. Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity.

Original language	English
Pages (from-to)	97-105
Number of pages	9
Journal	Experimental Parasitology
Volume	122
Issue number	2
DOIs	https://doi.org/10.1016/j.exppara.2009.03.003
Publication status	Published - Jun 2009

Keywords

Chabaupain
Malaria
Plasmodium chabaudi
Recombinant cysteine proteases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.exppara.2009.03.003

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title = "Plasmodium chabaudi: Expression of active recombinant chabaupain-1 and localization studies in Anopheles sp.",

abstract = "Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction. Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity.",

keywords = "Chabaupain, Malaria, Plasmodium chabaudi, Recombinant cysteine proteases",

author = "Caldeira, {Rubina L.} and Gon{\c c}alves, {L{\'i}dia M.D.} and Martins, {Tiago M.} and Henrique Silveira and Carlos Novo and Ros{\'a}rio, {Virg{\'i}lio do} and Ana Domingos",

year = "2009",

month = jun,

doi = "10.1016/j.exppara.2009.03.003",

language = "English",

volume = "122",

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journal = "Experimental Parasitology",

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AU - Caldeira, Rubina L.

AU - Gonçalves, Lídia M.D.

AU - Martins, Tiago M.

AU - Silveira, Henrique

AU - Novo, Carlos

AU - Rosário, Virgílio do

AU - Domingos, Ana

PY - 2009/6

Y1 - 2009/6

N2 - Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction. Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity.

AB - Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction. Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity.

KW - Chabaupain

KW - Malaria

KW - Plasmodium chabaudi

KW - Recombinant cysteine proteases

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JF - Experimental Parasitology

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ER -

Plasmodium chabaudi: Expression of active recombinant chabaupain-1 and localization studies in Anopheles sp.

Abstract

Keywords

UN SDGs

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