Plasmodium chabaudi: Expression of active recombinant chabaupain-1 and localization studies in Anopheles sp.

Rubina L. Caldeira, Lídia M.D. Gonçalves, Tiago M. Martins, Henrique Silveira, Carlos Novo, Virgílio do Rosário, Ana Domingos

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction. Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity.

Original languageEnglish
Pages (from-to)97-105
Number of pages9
JournalExperimental Parasitology
Volume122
Issue number2
DOIs
Publication statusPublished - Jun 2009

Keywords

  • Chabaupain
  • Malaria
  • Plasmodium chabaudi
  • Recombinant cysteine proteases

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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