Plant DNA sequencing for phylogenetic analyses: from plants to sequences

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Abstract

DNA sequences are important sources of data for phylogenetic analysis. Nowadays, DNA sequencing is a routine technique in molecular biology laboratories. However, there are specific questions associated with project design and sequencing of plant samples for phylogenetic analysis, which may not be familiar to researchers starting in the field. This chapter gives an overview of methods and protocols involved in the sequencing of plant samples, including general recommendations on the selection of species/taxa and DNA regions to be sequenced, and field collection of plant samples. Protocols of plant sample preparation, DNA extraction, PCR and cloning, which are critical to the success of molecular phylogenetic projects, are described in detail. Common problems of sequencing (using the Sanger method) are also addressed. Possible applications of second-generation sequencing techniques in plant phylogenetics are briefly discussed. Finally, orientation on the preparation of sequence data for phylogenetic analyses and submission to public databases is also given.
Original languageUnknown
Title of host publicationNetwork Biology
EditorsG. Cagney, A. Emili
Place of PublicationS.l.
PublisherHumana Press
Pages183-235
ISBN (Print)978-1-61779-276-2
Publication statusPublished - 1 Jan 2011

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Number781

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