@article{eb3b17185ae14a159c7a889eef73b0cd,
title = "Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5",
abstract = "The left-right (LR) field recognizes the importance of the mechanism involving the calcium permeable channel Polycystin-2. However, whether the early LR symmetry breaking mechanism is exclusively via Polycystin-2 has not been tested. For that purpose, we need to be able to isolate the effects of decreasing the levels of Pkd2 protein from any eventual effects on flow dynamics. Here we demonstrate that curly-up (cup) homozygous mutants have abnormal flow dynamics. In addition, we performed one cell stage Pkd2 knockdowns and LR organizer specific Pkd2 knockdowns and observed that both techniques resulted in shorter cilia length and abnormal flow dynamics. We conclude that Pkd2 reduction leads to LR defects that cannot be assigned exclusively to its putative role in mediating mechanosensation because indirectly, by modifying cell shape or decreasing cilia length, Pkd2 deficit affects LR flow dynamics.",
keywords = "cilia length, dand5, flow dynamics, left-right, Pkd2, zebrafish",
author = "Raquel Jacinto and Pedro Sampaio and M{\'o}nica Roxo-Rosa and Sara Pestana and Lopes, {Susana S.}",
note = "Funding: This work was supported by the Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (FCT-ANR/BEX-BID/0153/2012 research grant). SL is funded by an FCT CEEC contract for Principal Investigator. The work was also supported by Sociedade Portuguesa de Nefrologia (research grant 2015), iNOVA4Health – UID/Multi/04462/2013 (a program financially supported by Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia/ Minist{\'e}rio da Educa{\c c}{\~a}o e Ci{\^e}ncia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement) and Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia (research grant – PTDC/BEXBID/1411/2014). MR-R was supported by national funds through Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia, in the context of the celebration of the program contract foreseen in the numbers 4, 5, and 6 of article 23 of D.L. no. 57/2016 of 29 August, as amended by Law no. 57/2017 of 19 July. Zebrafish used as animal model were reared and maintained in the CEDOC Fish Facility, with the support from the research consortia Congento, co-financed by Lisboa Regional Operational Program (Lisboa2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia",
year = "2021",
month = apr,
day = "1",
doi = "10.3389/fcell.2021.624531",
language = "English",
volume = "9",
journal = "Frontiers in Cell and Developmental Biology",
issn = "2296-634X",
publisher = "Frontiers Media",
}