Abstract
Asphodelus bento-rainhae subsp. bento-rainhae (Ab), an endemic species with relevant interest due to conservation concerns and Asphodelus macrocarpus subsp. macrocarpus (Am), are commonly known by the Portuguese name “abrótea” and their leaf (AbL, AmL), has been traditionally used for the treatment of ulcers, urinary and inflammatory disorders.
In this study, hydroethanolic extracts (70%) of dried leaf of both species were prepared and the main classes of secondary metabolites, including, total phenolic, flavonoid, anthraquinone, condensed and hydrolysable tannin and terpenoid contents were detected and quantified by spectrophotometric methods. Liquid-liquid (L-L) partition of crude extracts were obtained using ethyl ether (AbL-1, AmL-1), ethyl acetate (AbL-2, AmL-2) and water (AbL-3, AmL-3). Phytochemical screenings of all extracts were conducted using LC/ESI/MS and LC/UV-DAD co-chromatographic techniques. Moreover, in vitro determination of antioxidant activity by FRAP and DPPH assays and preliminary genotoxicity/carcinogenicity by Ames test were performed.
Twelve compounds, namely, neochlorogenic acid, chlorogenic acid, caffeic acid, ρ-coumaric acid, ferulic acid, isoorientin, isovitexin, luteolin, diosmetin, aloe-emodin, chrysophanol and β-sitosterol were identified as major constituents in both species. Among the species, AbL fractions showed stronger antioxidant activity in comparison to AmL fractions and among the fractions, AbL-2 (IC50: 0.8 mg/mL) and AmL-2 (IC50: 1.2 mg/mL) exhibited the highest activity when compared to all the other fractions. No genotoxicity/mutagenicity potential of crude extracts (up to 5 mg/plate, with/without metabolomic activation) was observed in both species.
The obtained results will contribute to quality evaluation and validation of the studied Asphodelus species and their future use as herbal medicines.
In this study, hydroethanolic extracts (70%) of dried leaf of both species were prepared and the main classes of secondary metabolites, including, total phenolic, flavonoid, anthraquinone, condensed and hydrolysable tannin and terpenoid contents were detected and quantified by spectrophotometric methods. Liquid-liquid (L-L) partition of crude extracts were obtained using ethyl ether (AbL-1, AmL-1), ethyl acetate (AbL-2, AmL-2) and water (AbL-3, AmL-3). Phytochemical screenings of all extracts were conducted using LC/ESI/MS and LC/UV-DAD co-chromatographic techniques. Moreover, in vitro determination of antioxidant activity by FRAP and DPPH assays and preliminary genotoxicity/carcinogenicity by Ames test were performed.
Twelve compounds, namely, neochlorogenic acid, chlorogenic acid, caffeic acid, ρ-coumaric acid, ferulic acid, isoorientin, isovitexin, luteolin, diosmetin, aloe-emodin, chrysophanol and β-sitosterol were identified as major constituents in both species. Among the species, AbL fractions showed stronger antioxidant activity in comparison to AmL fractions and among the fractions, AbL-2 (IC50: 0.8 mg/mL) and AmL-2 (IC50: 1.2 mg/mL) exhibited the highest activity when compared to all the other fractions. No genotoxicity/mutagenicity potential of crude extracts (up to 5 mg/plate, with/without metabolomic activation) was observed in both species.
The obtained results will contribute to quality evaluation and validation of the studied Asphodelus species and their future use as herbal medicines.
| Original language | English |
|---|---|
| Pages (from-to) | 1517-1517 |
| Journal | Planta Medica |
| Volume | 88 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - Dec 2022 |