Phycocyanin protects against Alpha-Synuclein toxicity in yeast

D. Macedo, T.E. Bertolin, T. Oro, L.T.H. Backes, I.C. Brás, C.N. Santos, S. Tenreiro, T.F. Outeiro

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Parkinson's disease (PD) is an age-related neurodegenerative disorder associated with the misfolding and aggregation of alpha-synuclein (aSyn) in proteinaceous inclusions. These inclusions are common to a wider spectrum of disorders known as synucleinopathies. While the molecular bases of PD are still unclear, oxidative stress and impairment of protein quality-control systems play an important role, and are regarded as valuable targets for therapeutic interventions. We employed a yeast model of synucleinopathies to test the cytoprotective potential of phycocyanin, a biliprotein used as a nutritional supplement. We found that phycocyanin effectively protected against aSyn toxicity in yeast and, importantly, the mechanism of action involves the reduction of aSyn inclusions and superoxide levels, through the regulation of genes and enzymes involved in oxidative stress response, glutathione metabolism, and cellular protein quality-control systems. Overall, this study highlights the potential of phycocyanin as a nutraceutical in neurodegenerative diseases associated with proteotoxicity, such as synucleinopathies. © 2017 Elsevier Ltd
Original languageEnglish
Pages (from-to)553-560
Number of pages8
JournalJournal of Functional Foods
Volume38
DOIs
Publication statusPublished - 2017

Fingerprint

Phycocyanin
alpha-Synuclein
Yeasts
Parkinson disease
neurodegenerative diseases
yeasts
toxicity
Neurodegenerative Diseases
Quality Control
quality control
Parkinson Disease
Oxidative Stress
oxidative stress
Dietary Supplements
Heat-Shock Proteins
Superoxides
superoxide anion
functional foods
Glutathione
dietary supplements

Keywords

  • Alpha-synuclein
  • Oxidative stress
  • Parkinson's disease
  • Phycocyanin
  • Proteostasis

Cite this

Macedo, D. ; Bertolin, T.E. ; Oro, T. ; Backes, L.T.H. ; Brás, I.C. ; Santos, C.N. ; Tenreiro, S. ; Outeiro, T.F. / Phycocyanin protects against Alpha-Synuclein toxicity in yeast. In: Journal of Functional Foods. 2017 ; Vol. 38. pp. 553-560.
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Phycocyanin protects against Alpha-Synuclein toxicity in yeast. / Macedo, D.; Bertolin, T.E.; Oro, T.; Backes, L.T.H.; Brás, I.C.; Santos, C.N.; Tenreiro, S.; Outeiro, T.F.

In: Journal of Functional Foods, Vol. 38, 2017, p. 553-560.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Phycocyanin protects against Alpha-Synuclein toxicity in yeast

AU - Macedo, D.

AU - Bertolin, T.E.

AU - Oro, T.

AU - Backes, L.T.H.

AU - Brás, I.C.

AU - Santos, C.N.

AU - Tenreiro, S.

AU - Outeiro, T.F.

PY - 2017

Y1 - 2017

N2 - Parkinson's disease (PD) is an age-related neurodegenerative disorder associated with the misfolding and aggregation of alpha-synuclein (aSyn) in proteinaceous inclusions. These inclusions are common to a wider spectrum of disorders known as synucleinopathies. While the molecular bases of PD are still unclear, oxidative stress and impairment of protein quality-control systems play an important role, and are regarded as valuable targets for therapeutic interventions. We employed a yeast model of synucleinopathies to test the cytoprotective potential of phycocyanin, a biliprotein used as a nutritional supplement. We found that phycocyanin effectively protected against aSyn toxicity in yeast and, importantly, the mechanism of action involves the reduction of aSyn inclusions and superoxide levels, through the regulation of genes and enzymes involved in oxidative stress response, glutathione metabolism, and cellular protein quality-control systems. Overall, this study highlights the potential of phycocyanin as a nutraceutical in neurodegenerative diseases associated with proteotoxicity, such as synucleinopathies. © 2017 Elsevier Ltd

AB - Parkinson's disease (PD) is an age-related neurodegenerative disorder associated with the misfolding and aggregation of alpha-synuclein (aSyn) in proteinaceous inclusions. These inclusions are common to a wider spectrum of disorders known as synucleinopathies. While the molecular bases of PD are still unclear, oxidative stress and impairment of protein quality-control systems play an important role, and are regarded as valuable targets for therapeutic interventions. We employed a yeast model of synucleinopathies to test the cytoprotective potential of phycocyanin, a biliprotein used as a nutritional supplement. We found that phycocyanin effectively protected against aSyn toxicity in yeast and, importantly, the mechanism of action involves the reduction of aSyn inclusions and superoxide levels, through the regulation of genes and enzymes involved in oxidative stress response, glutathione metabolism, and cellular protein quality-control systems. Overall, this study highlights the potential of phycocyanin as a nutraceutical in neurodegenerative diseases associated with proteotoxicity, such as synucleinopathies. © 2017 Elsevier Ltd

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KW - Oxidative stress

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