Phosphorylation status of BolA affects its role in transcription and biofilm development

Research output: Contribution to journalArticlepeer-review

Abstract

BolA has been characterized as an important transcriptional regulator, which is induced in stationary phase of growth, and in response to several stresses. In Escherichia coli, its cellular function is associated with cell wall synthesis and division, morphology, permeability, motility and biofilm formation. Phosphorylation has been widely described as one of the most important events involved in the modulation of the activity of many transcription factors. In the present work, we have demonstrated in vivo and by mass spectrometry that BolA is phosphorylated in four highly conserved protein positions: S26, S45, T81 and S95. S95 is located in the C terminus unstructured region of the protein, and the other three sites are in the DNA-binding domain. These positions were mutated to nonphosphorylated residues, and their effects were investigated on different known BolA functions. Using northern blot experiments, we showed that the regulation of the expression of these Ser/Thr BolA mutants is performed at the post-translational level. Western blot results revealed that the stability/turnover of the mutated BolA proteins is differently affected depending on the dephosphorylated residue. Moreover, we provide evidences that phosphorylation events are crucial in the modulation of BolA activity as a transcription factor and as a regulator of cell morphology and biofilm development. Here, we propose that phosphorylation affects BolA downstream functions and discuss the possible significance of these phosphoresidues in the protein structure, stability, dimerization and function as a transcription factor.

Original languageEnglish
Pages (from-to)961-979
Number of pages19
JournalFEBS Journal
Volume288
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • biofilm
  • BolA-like proteins
  • morphogene and phosphorylation
  • transcription factor

Fingerprint Dive into the research topics of 'Phosphorylation status of BolA affects its role in transcription and biofilm development'. Together they form a unique fingerprint.

Cite this