Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview

Íris L Batalha, Ana Cecília A Roque

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

13 Citations (Scopus)


Magnetic nanocomposites are hybrid structures consisting of an iron oxide (Fe3O4/γ-Fe2O3) superparamagnetic core and a coating shell which presents affinity for a specific target molecule. Within the scope of phosphopeptide enrichment, the magnetic core is usually first functionalized with an intermediate layer of silica or carbon to improve dispersibility and increase specific area, and then with an outer layer of a phosphate-affinity material. Fe3O4-coating materials include metal oxides, rare earth metal-based compounds, immobilized-metal ions, polymers, and many others. This chapter provides a generic overview of the different materials that can be found in literature and their advantages and drawbacks.

Original languageEnglish
Title of host publicationPhospho-Proteomics
Number of pages17
Publication statusPublished - 2016

Publication series

Name Methods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Aluminum Oxide
  • Animals
  • Ferrosoferric Oxide
  • Humans
  • Magnetite Nanoparticles
  • Nanotechnology
  • Peptide Mapping
  • Phosphopeptides
  • Phosphorylation
  • Polymers
  • Protein Processing, Post-Translational
  • Proteomics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Titanium
  • Zirconium
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Review


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