Phosphoneurofilament heavy chain and N-glycomics from the cerebrospinal fluid in amyotrophic lateral sclerosis

Margarida Gonçalves, Linda Tillack, Mamede de Carvalho, Susana Pinto, Harald S. Conradt, Júlia Costa

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Background: Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease of the motor neuron for which no clinically validated biomarkers have been identified. Methods: We have quantified by ELISA the biomarker phosphoneurofilament heavy chain (pNFH) in the cerebrospinal fluid (CSF) of ALS patients (n. =. 29) and age-matched control patients with other diseases (n. =. 19) by ELISA. Furthermore, we compared protein N-glycosylation of the CSF in ALS patients and controls, by applying a glycomics approach based on liquid chromatography and mass spectrometry. Results: pNFH levels were significantly higher in ALS patients in comparison with controls (P.

Original languageEnglish
Pages (from-to)342-349
Number of pages8
JournalClinica chimica acta; international journal of clinical chemistry
Volume438
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Amyotrophic lateral sclerosis
  • Biomarker
  • Cerebrospinal fluid
  • Glycomics
  • Glycoproteins
  • Phosphoneurofilament heavy chain

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