TY - JOUR
T1 - Phenolic compounds from Nerium oleander leaves
T2 - Microwave assisted extraction, characterization, antiproliferative and cytotoxic activities
AU - Ayouaz, Siham
AU - Oliveira-Alves, Sheila Cristina
AU - Lefsih, Khalef
AU - Serra, Ana Teresa
AU - Bento Da Silva, Andreia
AU - Samah, Madani
AU - Karczewski, Jakub
AU - Madani, Khodir
AU - Bronze, Maria Rosário
PY - 2020/7
Y1 - 2020/7
N2 - A microwave-assisted extraction (MAE) method was used for the extraction of phenolic compounds from Nerium oleander leaves. The influence of variables such as ethanol concentration, microwave power, irradiation time and liquid/solid ratio on polyphenol extraction was modelled using a second-order regression equation based on response surface methodology (RSM). The optimal conditions for MAE were: extraction solvent 35% ethanol concentration, 500 W microwave power, 60 s irradiation time and a solvent/material ratio of 20 mL g-1. Under optimal MAE conditions, the recovery of TPC was 25.752 mg GAE per g dw. 19 compounds have been identified by HPLC-ESI-MS/MS analysis; the main compounds identified were chlorogenic acid, rutin and quinic acid esters, such as caffeoylquinic acids and dicaffeoylquinic acids. Additionally, the optimized extract demonstrated potential to inhibit HT29 colorectal cancer cell growth (EC50 = 2.432 µg mL-1) without presenting cytotoxicity in confluent Caco-2 cells, a model of human intestinal epithelium. These results supply new information about the phenolic composition of Nerium oleander leaves and their antiproliferative effect.
AB - A microwave-assisted extraction (MAE) method was used for the extraction of phenolic compounds from Nerium oleander leaves. The influence of variables such as ethanol concentration, microwave power, irradiation time and liquid/solid ratio on polyphenol extraction was modelled using a second-order regression equation based on response surface methodology (RSM). The optimal conditions for MAE were: extraction solvent 35% ethanol concentration, 500 W microwave power, 60 s irradiation time and a solvent/material ratio of 20 mL g-1. Under optimal MAE conditions, the recovery of TPC was 25.752 mg GAE per g dw. 19 compounds have been identified by HPLC-ESI-MS/MS analysis; the main compounds identified were chlorogenic acid, rutin and quinic acid esters, such as caffeoylquinic acids and dicaffeoylquinic acids. Additionally, the optimized extract demonstrated potential to inhibit HT29 colorectal cancer cell growth (EC50 = 2.432 µg mL-1) without presenting cytotoxicity in confluent Caco-2 cells, a model of human intestinal epithelium. These results supply new information about the phenolic composition of Nerium oleander leaves and their antiproliferative effect.
UR - http://www.scopus.com/inward/record.url?scp=85088488593&partnerID=8YFLogxK
U2 - 10.1039/d0fo01180k
DO - 10.1039/d0fo01180k
M3 - Article
C2 - 32608462
AN - SCOPUS:85088488593
SN - 2042-6496
VL - 11
SP - 6319
EP - 6331
JO - Food and Function
JF - Food and Function
IS - 7
ER -
