Pharmacokinetics of blackberry anthocyanins consumed with or without ethanol: A randomized and crossover trial

Cláudia Marques; Iva Fernandes; Sónia Norberto; Carla Sá; Diana Teixeira; Victor de Freitas; Nuno Mateus; Conceição Calhau; Ana Faria

doi:10.1002/mnfr.201600143

Pharmacokinetics of blackberry anthocyanins consumed with or without ethanol: A randomized and crossover trial

Cláudia Marques, Iva Fernandes, Sónia Norberto, Carla Sá, Diana Teixeira, Victor de Freitas, Nuno Mateus, Conceição Calhau, Ana Faria

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Abstract

Scope: This study was designed to evaluate the influence of ethanol on the bioavailability of blackberry anthocyanins. Methods and results: A total of 18 participants were recruited to consume 250 mL of a blackberry puree (650 mg of anthocyanins) without (BBP) or with 12% ethanol (BBP 12%). Venous blood was collected from participants at baseline and at 15, 30, 60, and 120 min after puree ingestion. Urine samples were collected at baseline and at 120 min. Plasma and urine concentration of anthocyanins and anthocyanin conjugates were quantified by HPLC-DAD. Methyl-cyanidin-glucuronide (Me-Cy-Glucr) and 3′-methyl-cyanidin-3-glucoside (3′-Me-Cy3glc) were the main anthocyanin conjugates detected in all plasma and urine samples. Urinary concentration of these anthocyanin conjugates were positively correlated with their plasma concentration. Ethanol increased plasma C_max of Me-Cy-Glucr and 3′-Me-Cy3glc. Participants were then stratified according to their body mass index (BMI) and body fat mass. After BBP consumption, plasma C_max of Me-Cy-Glucr and 3′-Me-Cy3glc tended to be decreased in overweight/obese participants, in comparison to normal weight participants. The increase on plasma C_max of Me-Cy-Glucr and 3′-Me-Cy3glc induced by ethanol was more pronounced in the group of overweight/obese participants. Conclusions: Ethanol seems to enhance Cy3glc metabolism that appears to be compromised in overweight and obese individuals.

Original language	English
Pages (from-to)	2319-2330
Number of pages	12
Journal	Molecular Nutrition and Food Research
Volume	60
Issue number	11
DOIs	https://doi.org/10.1002/mnfr.201600143
Publication status	Published - 1 Nov 2016

Keywords

Anthocyanins
Bioavailability
Ethanol
Metabolites
Obesity

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10.1002/mnfr.201600143

Molecular Nutrition Food Res - 2016 - Marques - Pharmacokinetics of blackberry anthocyanins consumed with or withoutFinal published version, 856 KBLicence: CC BY

Cite this

@article{01144e0f7e45444c80cf8b41053275dc,

title = "Pharmacokinetics of blackberry anthocyanins consumed with or without ethanol: A randomized and crossover trial",

abstract = "Scope: This study was designed to evaluate the influence of ethanol on the bioavailability of blackberry anthocyanins. Methods and results: A total of 18 participants were recruited to consume 250 mL of a blackberry puree (650 mg of anthocyanins) without (BBP) or with 12% ethanol (BBP 12%). Venous blood was collected from participants at baseline and at 15, 30, 60, and 120 min after puree ingestion. Urine samples were collected at baseline and at 120 min. Plasma and urine concentration of anthocyanins and anthocyanin conjugates were quantified by HPLC-DAD. Methyl-cyanidin-glucuronide (Me-Cy-Glucr) and 3′-methyl-cyanidin-3-glucoside (3′-Me-Cy3glc) were the main anthocyanin conjugates detected in all plasma and urine samples. Urinary concentration of these anthocyanin conjugates were positively correlated with their plasma concentration. Ethanol increased plasma Cmax of Me-Cy-Glucr and 3′-Me-Cy3glc. Participants were then stratified according to their body mass index (BMI) and body fat mass. After BBP consumption, plasma Cmax of Me-Cy-Glucr and 3′-Me-Cy3glc tended to be decreased in overweight/obese participants, in comparison to normal weight participants. The increase on plasma Cmax of Me-Cy-Glucr and 3′-Me-Cy3glc induced by ethanol was more pronounced in the group of overweight/obese participants. Conclusions: Ethanol seems to enhance Cy3glc metabolism that appears to be compromised in overweight and obese individuals.",

keywords = "Anthocyanins, Bioavailability, Ethanol, Metabolites, Obesity",

author = "Cl{\'a}udia Marques and Iva Fernandes and S{\'o}nia Norberto and Carla S{\'a} and Diana Teixeira and {de Freitas}, Victor and Nuno Mateus and Concei{\c c}{\~a}o Calhau and Ana Faria",

note = "Funding Information: The commitment of all volunteers participating in the study is gratefully acknowledged. The contribution of Professor Jo{\~a}o Tiago Guimar{\~a}es and the technicians from the Department of Clinical Pathology (Hospital S. Jo{\~a}o) with plasma biochemical analysis is also gratefully acknowledged. The assistance of Dr. Renata Silva and Dr. Paula Guedes from Faculty of Pharmacy of the University of Porto with the MRM analysis of plasma and urine samples is also gratefully acknowledged. The authors must also recognize the commitment, support, and excellent assistance of Daniela Teixeira, the nurse involved in the study. Financial support from Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia (FCT) - PTDC/AGR-TEC/2227/2012, SFRH/BD/93073/2013, SFRH/BPD/75294/2010, and SFRH/BPD/86173/2012 - is gratefully acknowledged. Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2016",

month = nov,

day = "1",

doi = "10.1002/mnfr.201600143",

language = "English",

volume = "60",

pages = "2319--2330",

journal = "Molecular Nutrition and Food Research",

issn = "1613-4125",

publisher = "John Wiley & Sons, Ltd.",

number = "11",

}

TY - JOUR

T1 - Pharmacokinetics of blackberry anthocyanins consumed with or without ethanol

T2 - A randomized and crossover trial

AU - Marques, Cláudia

AU - Fernandes, Iva

AU - Norberto, Sónia

AU - Sá, Carla

AU - Teixeira, Diana

AU - de Freitas, Victor

AU - Mateus, Nuno

AU - Calhau, Conceição

AU - Faria, Ana

N1 - Funding Information: The commitment of all volunteers participating in the study is gratefully acknowledged. The contribution of Professor João Tiago Guimarães and the technicians from the Department of Clinical Pathology (Hospital S. João) with plasma biochemical analysis is also gratefully acknowledged. The assistance of Dr. Renata Silva and Dr. Paula Guedes from Faculty of Pharmacy of the University of Porto with the MRM analysis of plasma and urine samples is also gratefully acknowledged. The authors must also recognize the commitment, support, and excellent assistance of Daniela Teixeira, the nurse involved in the study. Financial support from Fundação para a Ciência e Tecnologia (FCT) - PTDC/AGR-TEC/2227/2012, SFRH/BD/93073/2013, SFRH/BPD/75294/2010, and SFRH/BPD/86173/2012 - is gratefully acknowledged. Publisher Copyright: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Scope: This study was designed to evaluate the influence of ethanol on the bioavailability of blackberry anthocyanins. Methods and results: A total of 18 participants were recruited to consume 250 mL of a blackberry puree (650 mg of anthocyanins) without (BBP) or with 12% ethanol (BBP 12%). Venous blood was collected from participants at baseline and at 15, 30, 60, and 120 min after puree ingestion. Urine samples were collected at baseline and at 120 min. Plasma and urine concentration of anthocyanins and anthocyanin conjugates were quantified by HPLC-DAD. Methyl-cyanidin-glucuronide (Me-Cy-Glucr) and 3′-methyl-cyanidin-3-glucoside (3′-Me-Cy3glc) were the main anthocyanin conjugates detected in all plasma and urine samples. Urinary concentration of these anthocyanin conjugates were positively correlated with their plasma concentration. Ethanol increased plasma Cmax of Me-Cy-Glucr and 3′-Me-Cy3glc. Participants were then stratified according to their body mass index (BMI) and body fat mass. After BBP consumption, plasma Cmax of Me-Cy-Glucr and 3′-Me-Cy3glc tended to be decreased in overweight/obese participants, in comparison to normal weight participants. The increase on plasma Cmax of Me-Cy-Glucr and 3′-Me-Cy3glc induced by ethanol was more pronounced in the group of overweight/obese participants. Conclusions: Ethanol seems to enhance Cy3glc metabolism that appears to be compromised in overweight and obese individuals.

AB - Scope: This study was designed to evaluate the influence of ethanol on the bioavailability of blackberry anthocyanins. Methods and results: A total of 18 participants were recruited to consume 250 mL of a blackberry puree (650 mg of anthocyanins) without (BBP) or with 12% ethanol (BBP 12%). Venous blood was collected from participants at baseline and at 15, 30, 60, and 120 min after puree ingestion. Urine samples were collected at baseline and at 120 min. Plasma and urine concentration of anthocyanins and anthocyanin conjugates were quantified by HPLC-DAD. Methyl-cyanidin-glucuronide (Me-Cy-Glucr) and 3′-methyl-cyanidin-3-glucoside (3′-Me-Cy3glc) were the main anthocyanin conjugates detected in all plasma and urine samples. Urinary concentration of these anthocyanin conjugates were positively correlated with their plasma concentration. Ethanol increased plasma Cmax of Me-Cy-Glucr and 3′-Me-Cy3glc. Participants were then stratified according to their body mass index (BMI) and body fat mass. After BBP consumption, plasma Cmax of Me-Cy-Glucr and 3′-Me-Cy3glc tended to be decreased in overweight/obese participants, in comparison to normal weight participants. The increase on plasma Cmax of Me-Cy-Glucr and 3′-Me-Cy3glc induced by ethanol was more pronounced in the group of overweight/obese participants. Conclusions: Ethanol seems to enhance Cy3glc metabolism that appears to be compromised in overweight and obese individuals.

KW - Anthocyanins

KW - Bioavailability

KW - Ethanol

KW - Metabolites

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=84981541467&partnerID=8YFLogxK

U2 - 10.1002/mnfr.201600143

DO - 10.1002/mnfr.201600143

M3 - Article

C2 - 27306520

AN - SCOPUS:84981541467

SN - 1613-4125

VL - 60

SP - 2319

EP - 2330

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

IS - 11

ER -

Pharmacokinetics of blackberry anthocyanins consumed with or without ethanol: A randomized and crossover trial

Abstract

Keywords

UN SDGs

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