TY - JOUR
T1 - pH-responsive phthalate cashew gum nanoparticles for improving drugs delivery and anti-Trypanosoma cruzi efficacy
AU - Oliveira, Antônia Carla de Jesus
AU - Silva, Emilliany Bárbara
AU - Oliveira, Thaisa Cardoso de
AU - Ribeiro, Fábio de Oliveira Silva
AU - Nadvorny, Daniella
AU - Oliveira, Johny Wysllas de Freitas
AU - Borrego-Sánchez, Ana
AU - Rodrigues, Klinger Antonio da Franca
AU - Silva, Marcelo Sousa
AU - Rolim-Neto, Pedro José
AU - Viseras, César
AU - Silva-Filho, Edson C.
AU - Silva, Durcilene Alves da
AU - Chaves, Luíse Lopes
AU - Soares, Mônica Felts de La Roca
AU - Soares-Sobrinho, José Lamartine
N1 - Funding Information:
The authors acknowledge Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco ( FACEPE ) for a scholarship. This study was supported by funding from the Spanish Group CTS-946 and project P18-RT-3786 .
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/3/1
Y1 - 2023/3/1
N2 - Nanotechnology is a crucial technology in recent years has resulted in new and creative applications of nanomedicine. Polymeric nanoparticles have increasing demands in pharmaceutical applications and require high reproducibility, homogeneity, and control over their properties. Work explores the use of cashew phthalate gum (PCG) as a particle-forming polymer. PCG exhibited a pH-sensitive behavior due to the of acid groups on its chains, and control drug release. We report the development of nanoparticles carrying benznidazole. Formulations were characterized by DLS, encapsulation efficiency, drug loading, FTIR, pH-responsive behavior, release, and in vitro kinetics. Interaction between polymer and drug was an evaluated by molecular dynamics. Morphology was observed by SEM, and in vitro cytotoxicity by MTT assay. Trypanocidal effect for epimastigote and trypomastigote forms was also evaluated. NPs responded to the slightly basic pH, triggering the release of BNZ. In acidic medium, they presented small size, spherical shape, and good stability. It was indicated NP with enhanced biological activity, reduced cytotoxicity, high anti T. cruzi performance, and pH-sensitive release. This work investigated properties related to the development and enhancement of nanoparticles. PCG has specific physicochemical properties that make it a promising alternative to drug delivery, however, there are still challenges to be overcome.
AB - Nanotechnology is a crucial technology in recent years has resulted in new and creative applications of nanomedicine. Polymeric nanoparticles have increasing demands in pharmaceutical applications and require high reproducibility, homogeneity, and control over their properties. Work explores the use of cashew phthalate gum (PCG) as a particle-forming polymer. PCG exhibited a pH-sensitive behavior due to the of acid groups on its chains, and control drug release. We report the development of nanoparticles carrying benznidazole. Formulations were characterized by DLS, encapsulation efficiency, drug loading, FTIR, pH-responsive behavior, release, and in vitro kinetics. Interaction between polymer and drug was an evaluated by molecular dynamics. Morphology was observed by SEM, and in vitro cytotoxicity by MTT assay. Trypanocidal effect for epimastigote and trypomastigote forms was also evaluated. NPs responded to the slightly basic pH, triggering the release of BNZ. In acidic medium, they presented small size, spherical shape, and good stability. It was indicated NP with enhanced biological activity, reduced cytotoxicity, high anti T. cruzi performance, and pH-sensitive release. This work investigated properties related to the development and enhancement of nanoparticles. PCG has specific physicochemical properties that make it a promising alternative to drug delivery, however, there are still challenges to be overcome.
KW - Anti-Trypanosoma cruzi
KW - Benznidazole
KW - Cashew gum polysaccharides
KW - Chemical modification
KW - Plackett-Burman design
KW - Polymeric nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85146442813&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.123272
DO - 10.1016/j.ijbiomac.2023.123272
M3 - Article
C2 - 36649864
AN - SCOPUS:85146442813
SN - 0141-8130
VL - 230
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 123272
ER -
