@article{33148f46dbba41559e15413ede285f36,
title = "Peroxidase-like activity of cytochrome b5 is triggered upon hemichrome formation in alkaline pH",
abstract = "In alkaline media (pH 12) a catalytic peroxidase activity of cytochrome b5 was found associated to a different conformational state. Upon incubation at this pH, cytochrome b5 electronic absorption spectrum was altered, with disappearance of characteristic bands of cytochrome b5 at pH 7.0. The appearance of new electronic absorption bands and EPR measurements support the formation of a cytochrome b5 class B hemichrome with an acquired ability to bind polar ligands. This hemichrome is characterized by a negative formal redox potential and the same folding properties than cytochrome b5 at pH 7. The acquired peroxidase-like activity of cytochrome b5 found at pH 12, driven by a hemichrome formation, suggests a role of this protein in peroxidation products propagation.",
keywords = "Alkaline pH, Cytochrome b, Hemichromes, Hydrogen peroxide, Low spin state, Peroxidase",
author = "Samhan-Arias, {Alejandro K.} and Maia, {Luisa B.} and Cordas, {Cristina M.} and Isabel Moura and Carlos Gutierrez-Merino and Moura, {Jos{\'e} J. G.}",
note = "info:eu-repo/grantAgreement/FCT/5876/147258/PT# Recombinant plasmid for human erythrocyte Cb<INF>5</INF> was kindly provided by Dr. A. Grant Mauk from the University of British Columbia, Vancouver, Canada. This work was supported by the Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO, which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). Experimental work was also partially supported by funding from Ayuda a Grupos de la Junta de Extremadura (GR15139 to Group BBB008) co-financed by the European Funds for Structural Development (FEDER). AMA and LBM also thank FCT/MCTES for the post-doctoral fellowship grants (SFRH/BPD/100069/2014 and SFRH/BPD/111404/2015, respectively), which are financed by national funds and co-financed by FSE.",
year = "2018",
month = feb,
day = "1",
doi = "10.1016/j.bbapap.2017.09.010",
language = "English",
volume = "1866",
pages = "373--378",
journal = "Biochimica et Biophysica Acta - Proteins and Proteomics",
issn = "1570-9639",
publisher = "Elsevier",
number = "2",
}