Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo

Catarina Roma-Rodrigues, Amelie Heuer-Jungemann, Maria Alexandra Núncio de Carvalho Ramos Fernandes, Antonios G. Kanaras, Pedro Miguel Ribeiro Viana Baptista

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In this work, peptides designed to selectively interact with cellular receptors involved in the regulation of angiogenesis were anchored to oligo-ethylene glycol-capped gold nanoparticles (AuNPs) and used to evaluate the modulation of vascular development using an ex ovo chick chorioallantoic membrane assay. These nanoparticles alter the balance between naturally secreted pro- and antiangiogenic factors, under various biological conditions, without causing toxicity. Exposure of chorioallantoic membranes to AuNP-peptide activators of angiogenesis accelerated the formation of new arterioles when compared to scrambled peptide-coated nanoparticles. On the other hand, antiangiogenic AuNP-peptide conjugates were able to selectively inhibit angiogenesis in vivo. We demonstrated that AuNP vectorization is crucial for enhancing the effect of active peptides. Our data showed for the first time the effective control of activation or inhibition of blood vessel formation in chick embryo via AuNP-based formulations suitable for the selective modulation of angiogenesis, which is of paramount importance in applications where promotion of vascular growth is desirable (eg, wound healing) or ought to be contravened, as in cancer development.

Original languageEnglish
Pages (from-to)2633-2639
Number of pages7
JournalInternational journal of nanomedicine
Volume11
DOIs
Publication statusPublished - 2016

Keywords

  • angiogenesis activators
  • antiangiogenic
  • CAM assay
  • gold nanoparticles
  • peptide-coated gold nanoparticles
  • vascular development
  • CELL-PENETRATING PEPTIDES
  • TARGETING ANGIOGENESIS
  • VITRO
  • EXOCYTOSIS
  • DELIVERY
  • GROWTH
  • LIGAND

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