Overexpressing Vitis vinifera YSK2 dehydrin in tobacco improves plant performance

Rahma Jardak-Jamoussi, Olfa Zarrouk, Asma Ben Salem, Néjia Zoghlami, Samiha Mejri, Samia Gandoura, Bilel Khiari, Ahmed Mliki, Manuela Chaves, Carla Pinheiro

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The constitutive expression of a Vitis vinifera dehydrin (DHN) of the YSK2 type (VvDhn) allowed for a more vigorous growth in transgenic tobacco plants than in wild-type (WT). In vitro, a very distinct germination kinetics and root development profile was observed both under optimal and stress conditions (control, mannitol, NaCl). Transgenic lines outperformed WT in germination success and precocity and root length. Furthermore, transgenic lines were able to germinate under 300 mM mannitol. Adult tobacco plants expressing VvDhn also displayed improved tolerance to both drought and salt stresses. The VvDhn lines under these constraints were significantly better than WT in terms of biomass and leaf number. Moreover, higher capacities to recover from drought were revealed. However, the major effect the VvDhn over-expression was observed under non limiting conditions. VvDhn interfered with the development program leading to higher biomass production (leaf, shoot and root) and to the production of higher number of leaves. The VvDhn gene show great promise for grapevine genetic improvement to drought and salinity tolerance. VvDhn gene can be used to screen and/or design for new grapevine rootstocks that together with viticultural management programs has the potential to cope successfully with a more adverse and unpredictable climate, which is highly demand by community.

Original languageEnglish
Pages (from-to)176-189
Number of pages14
JournalAgricultural Water Management
Volume164
DOIs
Publication statusPublished - 31 Jan 2016

Keywords

  • Biomass
  • Development
  • Drought tolerance improvement
  • Germination
  • Leaf number
  • Salinity tolerance improvement

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