TY - JOUR
T1 - Overexpressing Vitis vinifera YSK2 dehydrin in tobacco improves plant performance
AU - Jardak-Jamoussi, Rahma
AU - Zarrouk, Olfa
AU - Ben Salem, Asma
AU - Zoghlami, Néjia
AU - Mejri, Samiha
AU - Gandoura, Samia
AU - Khiari, Bilel
AU - Mliki, Ahmed
AU - Chaves, Manuela
AU - Pinheiro, Carla
PY - 2016/1/31
Y1 - 2016/1/31
N2 - The constitutive expression of a Vitis vinifera dehydrin (DHN) of the YSK2 type (VvDhn) allowed for a more vigorous growth in transgenic tobacco plants than in wild-type (WT). In vitro, a very distinct germination kinetics and root development profile was observed both under optimal and stress conditions (control, mannitol, NaCl). Transgenic lines outperformed WT in germination success and precocity and root length. Furthermore, transgenic lines were able to germinate under 300 mM mannitol. Adult tobacco plants expressing VvDhn also displayed improved tolerance to both drought and salt stresses. The VvDhn lines under these constraints were significantly better than WT in terms of biomass and leaf number. Moreover, higher capacities to recover from drought were revealed. However, the major effect the VvDhn over-expression was observed under non limiting conditions. VvDhn interfered with the development program leading to higher biomass production (leaf, shoot and root) and to the production of higher number of leaves. The VvDhn gene show great promise for grapevine genetic improvement to drought and salinity tolerance. VvDhn gene can be used to screen and/or design for new grapevine rootstocks that together with viticultural management programs has the potential to cope successfully with a more adverse and unpredictable climate, which is highly demand by community.
AB - The constitutive expression of a Vitis vinifera dehydrin (DHN) of the YSK2 type (VvDhn) allowed for a more vigorous growth in transgenic tobacco plants than in wild-type (WT). In vitro, a very distinct germination kinetics and root development profile was observed both under optimal and stress conditions (control, mannitol, NaCl). Transgenic lines outperformed WT in germination success and precocity and root length. Furthermore, transgenic lines were able to germinate under 300 mM mannitol. Adult tobacco plants expressing VvDhn also displayed improved tolerance to both drought and salt stresses. The VvDhn lines under these constraints were significantly better than WT in terms of biomass and leaf number. Moreover, higher capacities to recover from drought were revealed. However, the major effect the VvDhn over-expression was observed under non limiting conditions. VvDhn interfered with the development program leading to higher biomass production (leaf, shoot and root) and to the production of higher number of leaves. The VvDhn gene show great promise for grapevine genetic improvement to drought and salinity tolerance. VvDhn gene can be used to screen and/or design for new grapevine rootstocks that together with viticultural management programs has the potential to cope successfully with a more adverse and unpredictable climate, which is highly demand by community.
KW - Biomass
KW - Development
KW - Drought tolerance improvement
KW - Germination
KW - Leaf number
KW - Salinity tolerance improvement
UR - http://www.scopus.com/inward/record.url?scp=84952769179&partnerID=8YFLogxK
U2 - 10.1016/j.agwat.2015.09.023
DO - 10.1016/j.agwat.2015.09.023
M3 - Article
AN - SCOPUS:84952769179
VL - 164
SP - 176
EP - 189
JO - Agricultural Water Management
JF - Agricultural Water Management
SN - 0378-3774
ER -