TY - JOUR
T1 - Oryzisolibacter propanilivorax gen. Nov., sp. nov., a propanildegrading bacterium
AU - Vaz-Moreira, Ivone
AU - Narciso-da-rocha, Carlos
AU - Lopes, Ana Rita
AU - Carvalho, Gilda
AU - Lobo-da-Cunha, Alexandre
AU - Whitman, William B.
AU - Snauwaert, Cindy
AU - Vandamme, Peter
AU - Manaia, Célia M.
AU - Nunes, Olga C.
N1 - sem pdf conforme despacho.
Projects UID/Multi/50016/2013-CBQF and POCI-01-0145-FEDER-006939-LEPABE funded by FEDER funds through COMPETE2020 - Programa Operacional Competitividade e Internacionalizacao (POCI) - and by national funds through Fundacao para a Ciencia e a Tecnologia (FCT). IVM, CNR and ARL acknowledge FCT grants SFRH/BPD/87360/2012, SFRH/BD/97131/2013 and SFRH/BPD/92894/2013.
Office of Science of the US Department of Energy under Contract No. DE-AC02-05CH11231.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Strain EPL6T, a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6T was observed to be a member of the family Comamonadaceae, sharing the highest pairwise identity with type strains of the species Alicycliphilus denitrificans K601T (96.8 %) and Melaminivora alkalimesophila CY1T (96.8 %). Strain EPL6T was able to grow in a temperature range of 15-37 °C, pH 6-9 and in the presence of up to 4 % (w/v) NaCl and tested positive for catalase and oxidase reactions. The major respiratory quinone was Q 8. The genomic DNA had a G+C content of 69.4±0.9 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the major fatty acid methyl esters comprised C16: 0, C18: 1ω7c and summed feature 3 (C16: 1ω7c/iso-C15: 02-OH). Comparison of the genome sequence of strain EPL6T and of its closest neighbours, Melaminivora alkalimesophila CY1T and Alicycliphilus denitrificans K601T, yielded values of ANI ≤84.1 % and of AAI ≤80.3 %. Therefore, the genetic, phylogenetic, phenotypic and chemotaxonomic characteristics support the classification of this organism into a new taxon. Considering the genetic divergence of strain EPL6T from the type strains of the closest species, which belong to distinct genera, we propose a new genus within the family Comamonadaceae, named Oryzisolibacter propanilivorax gen. nov., sp. nov., represented by the isolate EPL6T as the type strain of the species (=LMG 28427T=CECT 8927T).
AB - Strain EPL6T, a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6T was observed to be a member of the family Comamonadaceae, sharing the highest pairwise identity with type strains of the species Alicycliphilus denitrificans K601T (96.8 %) and Melaminivora alkalimesophila CY1T (96.8 %). Strain EPL6T was able to grow in a temperature range of 15-37 °C, pH 6-9 and in the presence of up to 4 % (w/v) NaCl and tested positive for catalase and oxidase reactions. The major respiratory quinone was Q 8. The genomic DNA had a G+C content of 69.4±0.9 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the major fatty acid methyl esters comprised C16: 0, C18: 1ω7c and summed feature 3 (C16: 1ω7c/iso-C15: 02-OH). Comparison of the genome sequence of strain EPL6T and of its closest neighbours, Melaminivora alkalimesophila CY1T and Alicycliphilus denitrificans K601T, yielded values of ANI ≤84.1 % and of AAI ≤80.3 %. Therefore, the genetic, phylogenetic, phenotypic and chemotaxonomic characteristics support the classification of this organism into a new taxon. Considering the genetic divergence of strain EPL6T from the type strains of the closest species, which belong to distinct genera, we propose a new genus within the family Comamonadaceae, named Oryzisolibacter propanilivorax gen. nov., sp. nov., represented by the isolate EPL6T as the type strain of the species (=LMG 28427T=CECT 8927T).
KW - Comamonadaceae
KW - Herbicide degrader
KW - New genus
KW - Rice field soil
UR - http://www.scopus.com/inward/record.url?scp=85031781579&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002184
DO - 10.1099/ijsem.0.002184
M3 - Article
C2 - 28895512
AN - SCOPUS:85031781579
SN - 1466-5026
VL - 67
SP - 3752
EP - 3758
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 10
M1 - 002184
ER -