Orientation of the axial ligands and magnetic properties of the hemes in the cytochrome c 7 family from Geobacter sulfurreducens determined by paramagnetic NMR

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Abstract

Geobacter sulfurreducens is a sediment bacterium that contains a large number of multiheme cytochromes. The family of five c 7 triheme periplasmic cytochromes from Geobacter sulfurreducens shows structural diversity of the heme core. Structural characterization of the relative orientation of the axial ligands of these proteins by 13C-paramagnetic NMR was carried out. The structures in solution were compared with those obtained by X-ray crystallography. For some hemes significant differences exist between the two methods such that orientation of the magnetic axes obtained from NMR data and the orientation taken from the X-ray coordinates differ. The results allowed the orientation of the magnetic axes to be defined confidently with respect to the heme frame in solution, a necessary step for the use of paramagnetic constraints to improve the complete solution structure of these proteins.

Original languageEnglish
Pages (from-to)12713-12718
Number of pages6
JournalDalton Transactions
Volume40
Issue number47
DOIs
Publication statusPublished - 21 Dec 2011

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