@article{cb7f54b66e654c69a2ea4189a7946272,
title = "Orientation of the axial ligands and magnetic properties of the hemes in the cytochrome c 7 family from Geobacter sulfurreducens determined by paramagnetic NMR",
abstract = "Geobacter sulfurreducens is a sediment bacterium that contains a large number of multiheme cytochromes. The family of five c 7 triheme periplasmic cytochromes from Geobacter sulfurreducens shows structural diversity of the heme core. Structural characterization of the relative orientation of the axial ligands of these proteins by 13C-paramagnetic NMR was carried out. The structures in solution were compared with those obtained by X-ray crystallography. For some hemes significant differences exist between the two methods such that orientation of the magnetic axes obtained from NMR data and the orientation taken from the X-ray coordinates differ. The results allowed the orientation of the magnetic axes to be defined confidently with respect to the heme frame in solution, a necessary step for the use of paramagnetic constraints to improve the complete solution structure of these proteins.",
author = "Dantas, {Joana M.} and Saraiva, {Ivo H.} and Leonor Morgado and Silva, {Marta A.} and Marianne Schiffer and Salgueiro, {Carlos A.} and Louro, {Ricardo O.}",
note = "info:eu-repo/grantAgreement/FCT/3599-PPCDT/70182/PT# info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F36582%2F2007/PT# info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F61952%2F2009/PT# MIT-Pt/BSBB/0014/2008 (R.O.L.). I.H.S., Projecto deRe-equipamento Cientifico contract REDE/1517/RMN/2005, Portugal. The work at Argonne National Laboratory was supported by the U.S. Department of Energy's Office of Science, Biological and Environmental Research GTL program under contract No. DE-AC02-06CH11357. This work is a part of collaboration with Prof. D.R. Lovley (University of Massachusetts, Amherst) under the Genomics: GTL project.",
year = "2011",
month = dec,
day = "21",
doi = "10.1039/c1dt10975h",
language = "English",
volume = "40",
pages = "12713--12718",
journal = "Dalton Transactions",
issn = "1477-9226",
publisher = "RSC - Royal Society of Chemistry",
number = "47",
}