Abstract
Electrochemical SEIRA is the method of choice for characterizing a lipid-bilayer-anchored, membrane bound hydrogenase Immobilized on a gold electrode. This setup allows the study of the enzyme under conditions mimicking its natural environment. A single experiment provides all of the crucial spectroscopic information relating to she protein orientation; active Site of the protein, and the lipid bilayer and also direct electrochemical determination of the catalytic H-2 oxidation by the enzyme
Original language | Unknown |
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Pages (from-to) | 2794-2798 |
Journal | JOURNAL OF PHYSICAL CHEMISTRY LETTERS |
Volume | 4 |
Issue number | 17 |
DOIs | |
Publication status | Published - 1 Jan 2013 |