TY - JOUR
T1 - Optimisation of glycogen quantification in mixed microbial cultures
AU - Lanham, Ana B.
AU - Ricardo, Ana R.
AU - Coma, Marta
AU - Fradinho, Joana
AU - Carvalheira, Mónica
AU - Oehmen, Adrian
AU - Carvalho, Gilda
AU - Reis, Maria A. M.
N1 - The authors are thankful for the financial support from Fundacao para a Ciencia e Tecnologia, Portugal through the PhD Grants SFRH/BD/25275/2005, SFRH/BD29477/2006, SFRH/BD42085/2007, SFRH/BD65113/2009, the Post-Doc Grant BDP/30800/2006 and the Research Projects PTDC/EBB-EBI/103147/2008, PEst-C/EQB/LA0006/2011 and PEst-OE/EQB/LA0004/2011. Marta Coma also wishes to thank the financial support of the Spanish Government through the Research Project MICINN-PT2009-0047 and the Luso-Espanhola integrated action E-94/10.
PY - 2012/8/1
Y1 - 2012/8/1
N2 - This study addressed the key factors affecting the extraction and quantification of glycogen from floccular and granular mixed microbial cultures collected from activated sludge, nutrient removal systems and photosynthetic consortiums: acid concentration, hydrolysis time and concentration of biomass in the hydrolysis. Response surface modelling indicated that 0.9M HCl and a biomass concentration of 1mgmL -1 were optimal conditions for performing acid hydrolysis. Floccular samples only needed a 2-h hydrolysis time whereas granular samples required as much as 5h. An intermediate 3h yielded an error of 10% compared to the results obtained with the hydrolysis times specifically tailored to the type of biomass and can thus be recommended as a practical compromise.
AB - This study addressed the key factors affecting the extraction and quantification of glycogen from floccular and granular mixed microbial cultures collected from activated sludge, nutrient removal systems and photosynthetic consortiums: acid concentration, hydrolysis time and concentration of biomass in the hydrolysis. Response surface modelling indicated that 0.9M HCl and a biomass concentration of 1mgmL -1 were optimal conditions for performing acid hydrolysis. Floccular samples only needed a 2-h hydrolysis time whereas granular samples required as much as 5h. An intermediate 3h yielded an error of 10% compared to the results obtained with the hydrolysis times specifically tailored to the type of biomass and can thus be recommended as a practical compromise.
KW - Carbohydrate analysis
KW - Design of experiments
KW - Floccular and granular sludge
KW - Glycogen quantification
KW - Response surface modelling
UR - http://www.scopus.com/inward/record.url?scp=84862295967&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2012.05.087
DO - 10.1016/j.biortech.2012.05.087
M3 - Article
C2 - 22717572
AN - SCOPUS:84862295967
SN - 0960-8524
VL - 118
SP - 518
EP - 525
JO - Bioresource Technology
JF - Bioresource Technology
ER -
