TY - JOUR
T1 - Optical fiber immunosensors based on surface plasmon resonance for the detection of Escherichia coli
AU - Oliveira, Sandro C.
AU - Soares, Simone
AU - Rodrigues, Andreia C. M.
AU - Gonçalves, Bárbara V.
AU - Soares, Amadeu M. V. M.
AU - Santos, Nuno
AU - Kumar, Santosh
AU - Almeida, Pedro
AU - Marques, Carlos
N1 - Funding Information:
info:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0006%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50011%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50011%2F2020/PT#
info:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0037%2F2020/PT#
info:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F50025%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50025%2F2020/PT
info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEEI-EEE%2F0415%2F2021/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT#
info:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F50017%2F2020/PT#
info:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0094%2F2020/PT#
info:eu-repo/grantAgreement/FCT/CEEC IND5ed/2022.04595.CEECIND%2FCP1720%2FCT0029/PT#
info:eu-repo/grantAgreement/FCT//UI%2FBD%2F153066%2F2022/PT#
This work is part of the project DepurD (MAR-01.03.01-FEAMP-0046), supported by Portugal and the European Union through MAR2020, Portugal2020, and FEAMP.
Publisher Copyright:
© 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
PY - 2024/3/11
Y1 - 2024/3/11
N2 - Every year, millions of people suffer some form of illness associated with the consumption of contaminated food. Escherichia coli (E. coli), found in the intestines of humans and other animals, is commonly associated with various diseases, due to the existence of pathogenic strains. Strict monitoring of food products for human consumption is essential to ensure public health, but traditional cell culture-based methods are associated with long waiting times and high costs. New approaches must be developed to achieve cheap, fast, and on-site monitoring. Thus, in this work, we developed optical fiber sensors based on surface plasmon resonance. Gold and cysteamine-coated fibers were functionalized with anti-E. coli antibody and tested using E. coli suspensions with concentrations ranging from 1 cell/mL to 105 cells/mL. An average logarithmic sensitivity of 0.21 ± 0.01 nm/log(cells/mL) was obtained for three independent assays. An additional assay revealed that including molybdenum disulfide resulted in an increase of approximately 50% in sensitivity. Specificity and selectivity were also evaluated, and the sensors were used to analyze contaminated water samples, which verified their promising applicability in the aquaculture field.
AB - Every year, millions of people suffer some form of illness associated with the consumption of contaminated food. Escherichia coli (E. coli), found in the intestines of humans and other animals, is commonly associated with various diseases, due to the existence of pathogenic strains. Strict monitoring of food products for human consumption is essential to ensure public health, but traditional cell culture-based methods are associated with long waiting times and high costs. New approaches must be developed to achieve cheap, fast, and on-site monitoring. Thus, in this work, we developed optical fiber sensors based on surface plasmon resonance. Gold and cysteamine-coated fibers were functionalized with anti-E. coli antibody and tested using E. coli suspensions with concentrations ranging from 1 cell/mL to 105 cells/mL. An average logarithmic sensitivity of 0.21 ± 0.01 nm/log(cells/mL) was obtained for three independent assays. An additional assay revealed that including molybdenum disulfide resulted in an increase of approximately 50% in sensitivity. Specificity and selectivity were also evaluated, and the sensors were used to analyze contaminated water samples, which verified their promising applicability in the aquaculture field.
UR - http://www.scopus.com/inward/record.url?scp=85187803786&partnerID=8YFLogxK
U2 - 10.1364/OE.518723
DO - 10.1364/OE.518723
M3 - Article
C2 - 38571228
AN - SCOPUS:85187803786
SN - 1094-4087
VL - 32
SP - 10077
EP - 10092
JO - Optics Express
JF - Optics Express
IS - 6
ER -