TY - JOUR
T1 - On the role of epigallocatechin-3-gallate in protecting phospholipid molecules against UV irradiation
AU - Pires, Filipa
AU - Geraldo, Vananelia P.N.
AU - Antunes, Andrea
AU - Marletta, Alexandre
AU - Oliveira, Osvaldo N.
AU - Raposo, Maria
N1 - info:eu-repo/grantAgreement/FCT/5876/147412/PT#
The authors acknowledge the financial support from FEDER, through Programa Operacional Factores de Competitividade - COMPETE and Fundacao para a Ciencia e a Tecnologia-FCT, by the project PTDC/FIS-NAN/0909/2014 and for the Portuguese research Grant No. PEst-OE/FIS/UI0068/2011 and UID/FIS/00068/2013 through FCT-MEC (Portugal) and by FAPESP (2013/14262-7) and CNPq (Brazil). Filipa Pires acknowledges the fellowship PD/BD/106036/2015 from RABBIT Doctoral Programme (Portugal).
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Catechin molecules such as epigallocatechin-3-gallate (EGCG) are capable of attenuating the biomolecular damage induced by UV radiation, possibly through molecular mechanisms involving the cell membranes. In this study, we confirmed the protective role of EGCG against UV of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol) (sodium salt) (DPPG) in liposomes and cast films. The incorporation of EGCG increased the stability of DPPG liposomes as indicated by UV–vis absorption spectra. Using 2D correlation spectroscopy to analyse the spectra, we found that DPPG and EGCG are co-helpers and complement each other against degradation induced by UV. At the molecular level, UV irradiation affects the phosphate and carbonyl groups of DPPG, in addition to triggering the oxidation and opening of the pyrogallol ring of EGCG. Since EGCG can be incorporated into liposomes and is a strong shield against UV radiation, one may envisage its use in anti-ageing and sunscreen creams, and in dermal drug delivery.
AB - Catechin molecules such as epigallocatechin-3-gallate (EGCG) are capable of attenuating the biomolecular damage induced by UV radiation, possibly through molecular mechanisms involving the cell membranes. In this study, we confirmed the protective role of EGCG against UV of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol) (sodium salt) (DPPG) in liposomes and cast films. The incorporation of EGCG increased the stability of DPPG liposomes as indicated by UV–vis absorption spectra. Using 2D correlation spectroscopy to analyse the spectra, we found that DPPG and EGCG are co-helpers and complement each other against degradation induced by UV. At the molecular level, UV irradiation affects the phosphate and carbonyl groups of DPPG, in addition to triggering the oxidation and opening of the pyrogallol ring of EGCG. Since EGCG can be incorporated into liposomes and is a strong shield against UV radiation, one may envisage its use in anti-ageing and sunscreen creams, and in dermal drug delivery.
KW - Catechins
KW - DPPG
KW - Drug delivery systems
KW - Liposome
KW - UV radiation
UR - http://www.scopus.com/inward/record.url?scp=85054384411&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2018.09.065
DO - 10.1016/j.colsurfb.2018.09.065
M3 - Article
C2 - 30308456
AN - SCOPUS:85054384411
SN - 0927-7765
VL - 173
SP - 312
EP - 319
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -