@article{7aae2ca1bde34dea8b04b3db8f6664bd,
title = "On the Mediated Electron Transfer of Immobilized Galactose Oxidase for Biotechnological Applications",
abstract = "The use of enzymes as catalysts in chemical synthesis offers advantages in terms of clean and highly selective transformations. Galactose oxidase (GalOx) is a remarkable enzyme with several applications in industrial conversions as it catalyzes the oxidation of primary alcohols. We have investigated the wiring of GalOx with a redox polymer; this enables mediated electron transfer with the electrode surface for its potential application in biotechnological conversions. As a result of electrochemical regeneration of the catalytic center, the formation of harmful H2O2 is minimized during enzymatic catalysis. The introduced bioelectrode was applied to the conversion of bio-renewable platform materials, with glycerol as model substrate. The biocatalytic transformations of glycerol and 5-hydroxymethylfurfural (HMF) were investigated in a circular flow-through setup to assess the possibility of substrate over-oxidation, which is observed for glycerol oxidation but not during HMF conversion.",
keywords = "bio-electrocatalysis, galactose oxidase, glycerol oxidation, modified electrodes, redox polymers",
author = "Fangyuan Zhao and Brix, {Ann Cathrin} and Anna Lielpetere and Wolfgang Schuhmann and Felipe Conzuelo",
note = "Funding Information: F.Z. is grateful for financial support from the Jiangsu Specially Appointed Professor Fund. The authors are grateful for financial support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) within the framework of the research unit FOR 2982 “UNODE–Unusual Anode Reactions” [413163866] and the SPP2240 (e-biotech) [445820469]. A.L. received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie MSCA-ITN “ImplantSens” [813006]. Open Access funding enabled and organized by Projekt DEAL. Funding Information: F.Z. is grateful for financial support from the Jiangsu Specially Appointed Professor Fund. The authors are grateful for financial support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) within the framework of the research unit FOR 2982 “UNODE–Unusual Anode Reactions” [413163866] and the SPP2240 (e‐biotech) [445820469]. A.L. received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sk{\l}odowska‐Curie MSCA‐ITN “ImplantSens” [813006]. Open Access funding enabled and organized by Projekt DEAL. Publisher Copyright: {\textcopyright} 2022 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.",
year = "2022",
month = may,
day = "25",
doi = "10.1002/chem.202200868",
language = "English",
volume = "28",
journal = "Chemistry - A European Journal",
issn = "0947-6539",
publisher = "Wiley",
number = "30",
}
