TY - JOUR
T1 - Oerskovia paurometabola can efficiently decolorize azo dye Acid Red 14 and remove its recalcitrant metabolite
AU - Franca, Rita Dias Guardão
AU - Vieira, Anabela
AU - Carvalho, Gilda
AU - Oehmen, Adrian
AU - Pinheiro, Helena Maria
AU - Barreto Crespo, Maria Teresa
AU - Lourenço, Nídia Dana
N1 - info:eu-repo/grantAgreement/FCT/5876/147338/PT#
info:eu-repo/grantAgreement/FCT/5876/147258/PT#
info:eu-repo/grantAgreement/FCT/3599-PPCDT/120624/PT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F95415%2F2013/PT#
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97640%2F2013/PT#
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F88095%2F2012/PT#
project PTDC/AAG-TEC/4501/2014.
FEDER (Fundo Europeu de Desenvolvimento Regional, Portugal) under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728, POCI-01-0145-FEDER-007728 and LISBOA-01-0145-FEDER-007344).
PY - 2020/3/15
Y1 - 2020/3/15
N2 - The biodegradation of dyes remains one of the biggest challenges of textile wastewater. Azo dyes are one of the most commonly employed dye classes, and biological treatment processes tend to generate recalcitrant aromatic amines, which are more toxic than the parent dye molecule. This study aimed to isolate bacterial strains with the capacity to degrade both the azo dye and the resulting aromatic amines towards the development of a simple and reliable treatment approach for dye-laden wastewaters. A mixed bacterial enrichment was first developed in an anaerobic-aerobic lab-scale sequencing batch reactor (SBR) fed with a synthetic textile wastewater containing the model textile azo dye Acid Red 14 (AR14). Eighteen bacterial strains were isolated from the SBR, including members of the Acinetobacter, Pseudomonas and Oerskovia genera, Oerskovia paurometabola presenting the highest decolorization capacity (91% after 24 h in static anaerobic culture). Growth assays supported that this is a facultative bacterium, and decolorization batch tests with 20–100 mg AR14 L−1 in a synthetic textile wastewater supplemented with yeast extract indicated that O. paurometabola has a high color removal capacity for a significant range of AR14 concentrations. In addition, a model typically used to describe biodegradation of xenobiotic compounds was adjusted to the results, to predict AR14 biodegradation time profiles at different initial concentrations. HPLC analysis confirmed that decolorization occurred through azo bond reduction under anaerobic conditions, the azo dye being completely reduced after 24 h of anaerobic incubation for the range of concentrations tested. Interestingly, partial (up to 63%) removal of one of the resulting aromatic amines (4-amino-naphthalene-1-sulfonic acid) was observed when subsequently subjected to aerobic conditions. Overall, this work showed the azo dye biodegradation potential of specific bacterial strains isolated from mixed culture bioreactors, reporting for the first time the decolorization capacity of an Oerskovia sp. with further biodegradation of a recalcitrant sulfonated aromatic amine metabolite.
AB - The biodegradation of dyes remains one of the biggest challenges of textile wastewater. Azo dyes are one of the most commonly employed dye classes, and biological treatment processes tend to generate recalcitrant aromatic amines, which are more toxic than the parent dye molecule. This study aimed to isolate bacterial strains with the capacity to degrade both the azo dye and the resulting aromatic amines towards the development of a simple and reliable treatment approach for dye-laden wastewaters. A mixed bacterial enrichment was first developed in an anaerobic-aerobic lab-scale sequencing batch reactor (SBR) fed with a synthetic textile wastewater containing the model textile azo dye Acid Red 14 (AR14). Eighteen bacterial strains were isolated from the SBR, including members of the Acinetobacter, Pseudomonas and Oerskovia genera, Oerskovia paurometabola presenting the highest decolorization capacity (91% after 24 h in static anaerobic culture). Growth assays supported that this is a facultative bacterium, and decolorization batch tests with 20–100 mg AR14 L−1 in a synthetic textile wastewater supplemented with yeast extract indicated that O. paurometabola has a high color removal capacity for a significant range of AR14 concentrations. In addition, a model typically used to describe biodegradation of xenobiotic compounds was adjusted to the results, to predict AR14 biodegradation time profiles at different initial concentrations. HPLC analysis confirmed that decolorization occurred through azo bond reduction under anaerobic conditions, the azo dye being completely reduced after 24 h of anaerobic incubation for the range of concentrations tested. Interestingly, partial (up to 63%) removal of one of the resulting aromatic amines (4-amino-naphthalene-1-sulfonic acid) was observed when subsequently subjected to aerobic conditions. Overall, this work showed the azo dye biodegradation potential of specific bacterial strains isolated from mixed culture bioreactors, reporting for the first time the decolorization capacity of an Oerskovia sp. with further biodegradation of a recalcitrant sulfonated aromatic amine metabolite.
KW - Aromatic amine biodegradation
KW - Azo dye biodegradation
KW - Decolorization kinetics
KW - Oerskovia sp.
KW - Textile wastewater
UR - http://www.scopus.com/inward/record.url?scp=85078764396&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2019.110007
DO - 10.1016/j.ecoenv.2019.110007
M3 - Article
C2 - 31796253
AN - SCOPUS:85078764396
VL - 191
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
SN - 0147-6513
M1 - 110007
ER -