TY - JOUR
T1 - Occupational second-hand smoke exposure
T2 - A comparative shotgun proteomics study on nasal epithelia from healthy restaurant workers
AU - Neves, Sofia
AU - Pacheco, Solange
AU - Vaz, Fátima
AU - James, Peter
AU - Simões, Tânia
AU - Penque, Deborah
N1 - Funding Information:
This work was supported by the Calouste Gulbenkian Foundation, Portuguese Central Administration of Nacional Health System, the Portuguese Rede Nacional de Espectrometria de Massa, the National Institute of Health Doctor Ricardo Jorge, and the Centre for Toxicogenomics and Human Health \u2013 ToxOmics from NOVA Medical School-FCM, UNL. SN and SP were granted with PhD fellowships from Portuguese Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia.
Funding Information:
This work was supported by the Calouste Gulbenkian Foundation, Portuguese Central Administration of National Health System, the Portuguese Rede Nacional de Espectrometria de Massa, the National Institute of Health Dr Ricardo Jorge, and the Centre for Toxicogenomics and Human Health \u2013 ToxOmics from NOVA Medical School-FCM, UNL. SN and SP were granted with PhD fellowships from Portuguese Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia.
Publisher Copyright:
© 2024 The Authors
PY - 2024/6
Y1 - 2024/6
N2 - Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure.
AB - Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure.
KW - Cigarette smoke
KW - Mass Spectrometry
KW - Nasal epithelium
KW - Protein network
KW - Proteomics
KW - Second-Hand Smoke
UR - http://www.scopus.com/inward/record.url?scp=85192172558&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2024.104459
DO - 10.1016/j.etap.2024.104459
M3 - Article
C2 - 38685369
AN - SCOPUS:85192172558
SN - 1382-6689
VL - 108
JO - Environmental toxicology and pharmacology
JF - Environmental toxicology and pharmacology
M1 - 104459
ER -
