Abstract
An inventory of culturable yeasts from the soil and water of natural CO2 springs (mofettes) in northeast Slovenia is presented. In mofettes, CO2 of geological origin reaches the soil surface causing temporarily and spatially stable hypoxic environments in soil and water. In total, 142 yeast strains were isolated and identified from high CO2 and control meadow soil, meadow ground-water, forest pond and stream water. All water locations showed below-ground CO2 release. They were assigned to six basidiomycetous yeast genera (six species) and 11 ascomycetous genera (18 species). All ascomycetous yeasts, with the exception of Debaryomyces hansenii, were able to grow under elevated CO2 and fermented glucose. Candida sophiae-reginae, Pichia fermentans and Candida vartiovaarae were the dominating species in meadow and forest high CO2 exposed water. Meyerozyma guilliermondii and Wickerhamomyces anomalus predominated in high CO2 exposed soils. Using high dilution plating of a mofette soil sample, four strains of an unknown basidiomycetous species were isolated and are here newly described as Occultifur mephitis based on molecular phylogenetic and phenotypic criteria. The type strain of Occultifur mephitis is EXF-6436T[CBS 14611=PYCC 7049, LT594852 (D1/D2), KX929055 (ITS)]. An additional three isolated strains are EXF-6437 (LT594853, KX929056), EXF-6473 (LT594863, KX929057) and EXF-6482 (LT594867, KX929054), as well as a strain reported from previous studies isolated from a leaf of Cistus albidus in Portugal (CBS 10223=PYCC 6067), EU002842 (D1/D2), KY308183 (ITS).
| Original language | English |
|---|---|
| Article number | 002824 |
| Pages (from-to) | 2285-2298 |
| Number of pages | 14 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 68 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1 Jul 2018 |
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Keywords
- Anoxia
- Biodiversity
- Carbon dioxide
- Ecology
- Fungi
- Occultifur mephitis
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Occultifur mephitis f.A., sp. Nov. and other yeast species from hypoxic and elevated CO2 mofette environments. / Šibanc, Nataša; Zalar, Polona; Schroers, Hans Josef; Zajc, Janja; Pontes, Ana; Sampaio, José Paulo; MaČEk, Irena.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 7, 002824, 01.07.2018, p. 2285-2298.Research output: Contribution to journal › Article
TY - JOUR
T1 - Occultifur mephitis f.A., sp. Nov. and other yeast species from hypoxic and elevated CO2 mofette environments
AU - Šibanc, Nataša
AU - Zalar, Polona
AU - Schroers, Hans Josef
AU - Zajc, Janja
AU - Pontes, Ana
AU - Sampaio, José Paulo
AU - MaČEk, Irena
N1 - info:eu-repo/grantAgreement/FCT/5876/147258/PT# Slovenian Research Agency projects (J4-2235, J4-5526, J4-7052), Slovenian Research Agency research core funding (P4-0085)
PY - 2018/7/1
Y1 - 2018/7/1
N2 - An inventory of culturable yeasts from the soil and water of natural CO2 springs (mofettes) in northeast Slovenia is presented. In mofettes, CO2 of geological origin reaches the soil surface causing temporarily and spatially stable hypoxic environments in soil and water. In total, 142 yeast strains were isolated and identified from high CO2 and control meadow soil, meadow ground-water, forest pond and stream water. All water locations showed below-ground CO2 release. They were assigned to six basidiomycetous yeast genera (six species) and 11 ascomycetous genera (18 species). All ascomycetous yeasts, with the exception of Debaryomyces hansenii, were able to grow under elevated CO2 and fermented glucose. Candida sophiae-reginae, Pichia fermentans and Candida vartiovaarae were the dominating species in meadow and forest high CO2 exposed water. Meyerozyma guilliermondii and Wickerhamomyces anomalus predominated in high CO2 exposed soils. Using high dilution plating of a mofette soil sample, four strains of an unknown basidiomycetous species were isolated and are here newly described as Occultifur mephitis based on molecular phylogenetic and phenotypic criteria. The type strain of Occultifur mephitis is EXF-6436T[CBS 14611=PYCC 7049, LT594852 (D1/D2), KX929055 (ITS)]. An additional three isolated strains are EXF-6437 (LT594853, KX929056), EXF-6473 (LT594863, KX929057) and EXF-6482 (LT594867, KX929054), as well as a strain reported from previous studies isolated from a leaf of Cistus albidus in Portugal (CBS 10223=PYCC 6067), EU002842 (D1/D2), KY308183 (ITS).
AB - An inventory of culturable yeasts from the soil and water of natural CO2 springs (mofettes) in northeast Slovenia is presented. In mofettes, CO2 of geological origin reaches the soil surface causing temporarily and spatially stable hypoxic environments in soil and water. In total, 142 yeast strains were isolated and identified from high CO2 and control meadow soil, meadow ground-water, forest pond and stream water. All water locations showed below-ground CO2 release. They were assigned to six basidiomycetous yeast genera (six species) and 11 ascomycetous genera (18 species). All ascomycetous yeasts, with the exception of Debaryomyces hansenii, were able to grow under elevated CO2 and fermented glucose. Candida sophiae-reginae, Pichia fermentans and Candida vartiovaarae were the dominating species in meadow and forest high CO2 exposed water. Meyerozyma guilliermondii and Wickerhamomyces anomalus predominated in high CO2 exposed soils. Using high dilution plating of a mofette soil sample, four strains of an unknown basidiomycetous species were isolated and are here newly described as Occultifur mephitis based on molecular phylogenetic and phenotypic criteria. The type strain of Occultifur mephitis is EXF-6436T[CBS 14611=PYCC 7049, LT594852 (D1/D2), KX929055 (ITS)]. An additional three isolated strains are EXF-6437 (LT594853, KX929056), EXF-6473 (LT594863, KX929057) and EXF-6482 (LT594867, KX929054), as well as a strain reported from previous studies isolated from a leaf of Cistus albidus in Portugal (CBS 10223=PYCC 6067), EU002842 (D1/D2), KY308183 (ITS).
KW - Anoxia
KW - Biodiversity
KW - Carbon dioxide
KW - Ecology
KW - Fungi
KW - Occultifur mephitis
UR - http://www.scopus.com/inward/record.url?scp=85049890774&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002824
DO - 10.1099/ijsem.0.002824
M3 - Article
VL - 68
SP - 2285
EP - 2298
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 7
M1 - 002824
ER -