Occultifur mephitis f.A., sp. Nov. and other yeast species from hypoxic and elevated CO2 mofette environments

Nataša Šibanc, Polona Zalar, Hans Josef Schroers, Janja Zajc, Ana Pontes, José Paulo Sampaio, Irena MaČEk

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An inventory of culturable yeasts from the soil and water of natural CO2 springs (mofettes) in northeast Slovenia is presented. In mofettes, CO2 of geological origin reaches the soil surface causing temporarily and spatially stable hypoxic environments in soil and water. In total, 142 yeast strains were isolated and identified from high CO2 and control meadow soil, meadow ground-water, forest pond and stream water. All water locations showed below-ground CO2 release. They were assigned to six basidiomycetous yeast genera (six species) and 11 ascomycetous genera (18 species). All ascomycetous yeasts, with the exception of Debaryomyces hansenii, were able to grow under elevated CO2 and fermented glucose. Candida sophiae-reginae, Pichia fermentans and Candida vartiovaarae were the dominating species in meadow and forest high CO2 exposed water. Meyerozyma guilliermondii and Wickerhamomyces anomalus predominated in high CO2 exposed soils. Using high dilution plating of a mofette soil sample, four strains of an unknown basidiomycetous species were isolated and are here newly described as Occultifur mephitis based on molecular phylogenetic and phenotypic criteria. The type strain of Occultifur mephitis is EXF-6436T[CBS 14611=PYCC 7049, LT594852 (D1/D2), KX929055 (ITS)]. An additional three isolated strains are EXF-6437 (LT594853, KX929056), EXF-6473 (LT594863, KX929057) and EXF-6482 (LT594867, KX929054), as well as a strain reported from previous studies isolated from a leaf of Cistus albidus in Portugal (CBS 10223=PYCC 6067), EU002842 (D1/D2), KY308183 (ITS).

Original languageEnglish
Article number002824
Pages (from-to)2285-2298
Number of pages14
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number7
DOIs
Publication statusPublished - 1 Jul 2018

Fingerprint

Mephitis
Mephitidae
yeast
Soil
Yeasts
yeasts
meadow
meadows
Water
Candida
soil
water
Pichia fermentans
Meyerozyma guilliermondii
Natural Springs
Wickerhamomyces anomalus
Cistus
Debaryomyces hansenii
Slovenia
edaphic factors

Keywords

  • Anoxia
  • Biodiversity
  • Carbon dioxide
  • Ecology
  • Fungi
  • Occultifur mephitis

Cite this

@article{1bc0078e172447f3841c10fac8c5deb3,
title = "Occultifur mephitis f.A., sp. Nov. and other yeast species from hypoxic and elevated CO2 mofette environments",
abstract = "An inventory of culturable yeasts from the soil and water of natural CO2 springs (mofettes) in northeast Slovenia is presented. In mofettes, CO2 of geological origin reaches the soil surface causing temporarily and spatially stable hypoxic environments in soil and water. In total, 142 yeast strains were isolated and identified from high CO2 and control meadow soil, meadow ground-water, forest pond and stream water. All water locations showed below-ground CO2 release. They were assigned to six basidiomycetous yeast genera (six species) and 11 ascomycetous genera (18 species). All ascomycetous yeasts, with the exception of Debaryomyces hansenii, were able to grow under elevated CO2 and fermented glucose. Candida sophiae-reginae, Pichia fermentans and Candida vartiovaarae were the dominating species in meadow and forest high CO2 exposed water. Meyerozyma guilliermondii and Wickerhamomyces anomalus predominated in high CO2 exposed soils. Using high dilution plating of a mofette soil sample, four strains of an unknown basidiomycetous species were isolated and are here newly described as Occultifur mephitis based on molecular phylogenetic and phenotypic criteria. The type strain of Occultifur mephitis is EXF-6436T[CBS 14611=PYCC 7049, LT594852 (D1/D2), KX929055 (ITS)]. An additional three isolated strains are EXF-6437 (LT594853, KX929056), EXF-6473 (LT594863, KX929057) and EXF-6482 (LT594867, KX929054), as well as a strain reported from previous studies isolated from a leaf of Cistus albidus in Portugal (CBS 10223=PYCC 6067), EU002842 (D1/D2), KY308183 (ITS).",
keywords = "Anoxia, Biodiversity, Carbon dioxide, Ecology, Fungi, Occultifur mephitis",
author = "Nataša Šibanc and Polona Zalar and Schroers, {Hans Josef} and Janja Zajc and Ana Pontes and Sampaio, {Jos{\'e} Paulo} and Irena MaČEk",
note = "info:eu-repo/grantAgreement/FCT/5876/147258/PT# Slovenian Research Agency projects (J4-2235, J4-5526, J4-7052), Slovenian Research Agency research core funding (P4-0085)",
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Occultifur mephitis f.A., sp. Nov. and other yeast species from hypoxic and elevated CO2 mofette environments. / Šibanc, Nataša; Zalar, Polona; Schroers, Hans Josef; Zajc, Janja; Pontes, Ana; Sampaio, José Paulo; MaČEk, Irena.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 7, 002824, 01.07.2018, p. 2285-2298.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Occultifur mephitis f.A., sp. Nov. and other yeast species from hypoxic and elevated CO2 mofette environments

AU - Šibanc, Nataša

AU - Zalar, Polona

AU - Schroers, Hans Josef

AU - Zajc, Janja

AU - Pontes, Ana

AU - Sampaio, José Paulo

AU - MaČEk, Irena

N1 - info:eu-repo/grantAgreement/FCT/5876/147258/PT# Slovenian Research Agency projects (J4-2235, J4-5526, J4-7052), Slovenian Research Agency research core funding (P4-0085)

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Y1 - 2018/7/1

N2 - An inventory of culturable yeasts from the soil and water of natural CO2 springs (mofettes) in northeast Slovenia is presented. In mofettes, CO2 of geological origin reaches the soil surface causing temporarily and spatially stable hypoxic environments in soil and water. In total, 142 yeast strains were isolated and identified from high CO2 and control meadow soil, meadow ground-water, forest pond and stream water. All water locations showed below-ground CO2 release. They were assigned to six basidiomycetous yeast genera (six species) and 11 ascomycetous genera (18 species). All ascomycetous yeasts, with the exception of Debaryomyces hansenii, were able to grow under elevated CO2 and fermented glucose. Candida sophiae-reginae, Pichia fermentans and Candida vartiovaarae were the dominating species in meadow and forest high CO2 exposed water. Meyerozyma guilliermondii and Wickerhamomyces anomalus predominated in high CO2 exposed soils. Using high dilution plating of a mofette soil sample, four strains of an unknown basidiomycetous species were isolated and are here newly described as Occultifur mephitis based on molecular phylogenetic and phenotypic criteria. The type strain of Occultifur mephitis is EXF-6436T[CBS 14611=PYCC 7049, LT594852 (D1/D2), KX929055 (ITS)]. An additional three isolated strains are EXF-6437 (LT594853, KX929056), EXF-6473 (LT594863, KX929057) and EXF-6482 (LT594867, KX929054), as well as a strain reported from previous studies isolated from a leaf of Cistus albidus in Portugal (CBS 10223=PYCC 6067), EU002842 (D1/D2), KY308183 (ITS).

AB - An inventory of culturable yeasts from the soil and water of natural CO2 springs (mofettes) in northeast Slovenia is presented. In mofettes, CO2 of geological origin reaches the soil surface causing temporarily and spatially stable hypoxic environments in soil and water. In total, 142 yeast strains were isolated and identified from high CO2 and control meadow soil, meadow ground-water, forest pond and stream water. All water locations showed below-ground CO2 release. They were assigned to six basidiomycetous yeast genera (six species) and 11 ascomycetous genera (18 species). All ascomycetous yeasts, with the exception of Debaryomyces hansenii, were able to grow under elevated CO2 and fermented glucose. Candida sophiae-reginae, Pichia fermentans and Candida vartiovaarae were the dominating species in meadow and forest high CO2 exposed water. Meyerozyma guilliermondii and Wickerhamomyces anomalus predominated in high CO2 exposed soils. Using high dilution plating of a mofette soil sample, four strains of an unknown basidiomycetous species were isolated and are here newly described as Occultifur mephitis based on molecular phylogenetic and phenotypic criteria. The type strain of Occultifur mephitis is EXF-6436T[CBS 14611=PYCC 7049, LT594852 (D1/D2), KX929055 (ITS)]. An additional three isolated strains are EXF-6437 (LT594853, KX929056), EXF-6473 (LT594863, KX929057) and EXF-6482 (LT594867, KX929054), as well as a strain reported from previous studies isolated from a leaf of Cistus albidus in Portugal (CBS 10223=PYCC 6067), EU002842 (D1/D2), KY308183 (ITS).

KW - Anoxia

KW - Biodiversity

KW - Carbon dioxide

KW - Ecology

KW - Fungi

KW - Occultifur mephitis

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U2 - 10.1099/ijsem.0.002824

DO - 10.1099/ijsem.0.002824

M3 - Article

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EP - 2298

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

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M1 - 002824

ER -