Novel methodology for quick detection of bacterial metabolites

Maria Gonçalves, Jorge Fernandes, Viktor Fetter, Mário Diniz, Valentina Vassilenko

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Fast detection of biological contaminations is of great importance in several areas of human health, such as, toxicology, environmental health and medicine. The need for developing sample retrieving methods in situ and techniques for fast and online detection of biological contamination is of crucial importance, not only for preventing infections but also to perform the most appropriate and effective medical treatment. In this work we report a method and an experimental protocol developed for the detection and analysis of VOCs emitted from bacteria by Ion Mobility Spectrometry coupled to Gas Chromatography (GC-IMS). As a biological model for study, the two Gram-Negative bacteria, Escherichia coli and Pseudomonas aeruginosa, were chosen. VOC profiles for these bacteria, individual and in the mixture, were obtained from the analysis of GC-IMS spectra. In addition, a study of the influence of the nutrition medium were performed.

Original languageEnglish
Title of host publication6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538685068
DOIs
Publication statusPublished - 15 Apr 2019
Event6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Lisbon, Portugal
Duration: 22 Feb 201923 Feb 2019

Conference

Conference6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019
CountryPortugal
CityLisbon
Period22/02/1923/02/19

Keywords

  • Escherichia coli identification
  • Gas Chromatography (GC)
  • Ion Mobility Spectrometry (IMS)
  • Pseudomonas aeruginosa identification
  • Volatile organic compound

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    Gonçalves, M., Fernandes, J., Fetter, V., Diniz, M., & Vassilenko, V. (2019). Novel methodology for quick detection of bacterial metabolites. In 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings [8692557] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ENBENG.2019.8692557