TY - JOUR
T1 - Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment
AU - Araújo, Diana
AU - Alves, Vítor D.
AU - Lima, Sofia A. C.
AU - Reis, Salette
AU - Freitas, Filomena
AU - Reis, Maria A. M.
N1 - UID/Multi/04378/2013 ,
UID/QUI/50006/2013
PEst-OE/AGR/UI0245/2014 ,
UID/QUI/50006/2019
POCI-01-0145-FEDER-007728
POCI-01-0145-FEDER-007265
grant BD/140829/2018 .
Sem PDF conforme despacho.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Chitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at −20 °C and thawing at room temperature; four cycles). The CGC solutions thus obtained were subjected to dialysis that induced the spontaneous gelation of the biopolymer, yielding translucid hydrogels with a yellowish coloration. Although all CGC hydrogels exhibited porous microstructures, high water content (above 97%) and good mechanical properties, their morphology, viscoelastic properties and texture were influenced by the type of solvent system used for CGC dissolution, as well as by their ionic strength. The K-based hydrogels presented a less compact network with larger pores and exhibited lower elastic properties. The Na-based hydrogels, on the other hand, exhibited a denser structure with smaller pores and a stiffer gel structure. These results show that it is possible to prepare CGC hydrogels with differing characteristics that can be suitable for different applications. Furthermore, all hydrogels were non-cytotoxic towards L929 fibroblasts and HaCaT keratinocytes. This study demonstrates CGC can be used to prepare biocompatible hydrogels with properties render them promising biomaterials.
AB - Chitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at −20 °C and thawing at room temperature; four cycles). The CGC solutions thus obtained were subjected to dialysis that induced the spontaneous gelation of the biopolymer, yielding translucid hydrogels with a yellowish coloration. Although all CGC hydrogels exhibited porous microstructures, high water content (above 97%) and good mechanical properties, their morphology, viscoelastic properties and texture were influenced by the type of solvent system used for CGC dissolution, as well as by their ionic strength. The K-based hydrogels presented a less compact network with larger pores and exhibited lower elastic properties. The Na-based hydrogels, on the other hand, exhibited a denser structure with smaller pores and a stiffer gel structure. These results show that it is possible to prepare CGC hydrogels with differing characteristics that can be suitable for different applications. Furthermore, all hydrogels were non-cytotoxic towards L929 fibroblasts and HaCaT keratinocytes. This study demonstrates CGC can be used to prepare biocompatible hydrogels with properties render them promising biomaterials.
KW - Biocompatibility
KW - Chitin-glucan complex
KW - Hydrogels
UR - http://www.scopus.com/inward/record.url?scp=85075984052&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2019.11.141
DO - 10.1016/j.ijbiomac.2019.11.141
M3 - Article
C2 - 31756470
AN - SCOPUS:85075984052
SN - 0141-8130
VL - 156
SP - 1104
EP - 1111
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -