NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens

Joana M. Dantas, Anton Brausemann, Oliver Einsle, Carlos A. Salgueiro

Research output: Contribution to journalLetter

5 Citations (Scopus)

Abstract

Geobacter sulfurreducens is a dissimilatory metal-reducing bacterium with notable properties and significance in biotechnological applications. Biochemical studies suggest that the inner membrane-associated diheme cytochrome MacA and the periplasmic triheme cytochrome PpcA from G. sulfurreducens can exchange electrons. In this work, NMR chemical shift perturbation measurements were used to map the interface region and to measure the binding affinity between PpcA and MacA. The results show that MacA binds to PpcA in a cleft defined by hemes I and IV, favoring the contact between PpcA heme IV and the MacA high-potential heme. The dissociation constant values indicate the formation of a low-affinity complex between the proteins, which is consistent with the transient interaction observed in electron transfer complexes.

Original languageEnglish
Pages (from-to)1657-1666
Number of pages10
JournalFEBS Letters
Volume591
Issue number12
DOIs
Publication statusPublished - 1 Jun 2017

Keywords

  • electron transfer
  • Geobacter
  • multiheme cytochromes
  • NMR
  • protein–protein interactions

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