TY - JOUR
T1 - Nitrous oxide reductase
AU - Pauleta, Sofia Rocha
AU - Dell'Acqua, Simone
AU - Moura, Isabel Maria Andrade Martins Galhardas de
N1 - Sem PDF.
We would like to thank the financial support provided by Fundacao para a Ciencia e Tecnologia to IM (PTDC/QUI-BIQ/116481/2010), SRP (PTDC/BIA-PRO/098882/2008) and to SD in the form of a Ph.D. scholarship (SFRH/BD/30414/2006), and by Conselho de Reitores Universidades Portuguesa - DAAD to SRP. The C.I.R.C.M.S.B. is gratefully acknowledged for a fellowship to SD.
PY - 2013
Y1 - 2013
N2 - Nitrous oxide is a potent greenhouse gas, whose atmospheric concentration has been increasing since the introduction of the Haber Bosch process led to the widespread use of nitrogenous fertilizers. One of the pathways to its destruction is reduction to molecular nitrogen by the enzyme nitrous oxide reductase found in denitrifying bacteria. This enzyme catalyzes the last step of the denitrification pathway. It has two copper centers, a binuclear CuA center, similar to the one found in cytochromecoxidase, and the CuZ center, a unique tetranuclear copper center now known to possess either one or two sulfide bridges. Nitrous oxide reductase has been isolated in different forms, depending on the oxidation state and molecular forms of its Cu centers. Recently, the structure of a purple form, which has both centers in the oxidized state, revealed that the CuZ center has the form [Cu4S2]. This review summarizes the biogenesis and regulation of nitrous oxide reductase, and the spectroscopic and kinetic properties of nitrous oxide reductase. The proposed activation and catalytic mechanism, as well as, electron transfer pathways are discussed in the light of the various structures of the CuZ center.
AB - Nitrous oxide is a potent greenhouse gas, whose atmospheric concentration has been increasing since the introduction of the Haber Bosch process led to the widespread use of nitrogenous fertilizers. One of the pathways to its destruction is reduction to molecular nitrogen by the enzyme nitrous oxide reductase found in denitrifying bacteria. This enzyme catalyzes the last step of the denitrification pathway. It has two copper centers, a binuclear CuA center, similar to the one found in cytochromecoxidase, and the CuZ center, a unique tetranuclear copper center now known to possess either one or two sulfide bridges. Nitrous oxide reductase has been isolated in different forms, depending on the oxidation state and molecular forms of its Cu centers. Recently, the structure of a purple form, which has both centers in the oxidized state, revealed that the CuZ center has the form [Cu4S2]. This review summarizes the biogenesis and regulation of nitrous oxide reductase, and the spectroscopic and kinetic properties of nitrous oxide reductase. The proposed activation and catalytic mechanism, as well as, electron transfer pathways are discussed in the light of the various structures of the CuZ center.
KW - CuZ center
KW - Nitrous oxide reductase
KW - Denitrification
KW - CuA center
KW - Nitrous oxide
KW - Copper-sulfide center
KW - Copper-sulfide center
KW - CuA center
KW - CuZ center
KW - Denitrification
KW - Nitrous oxide
KW - Nitrous oxide reductase
U2 - 10.1016/j.ccr.2012.05.026
DO - 10.1016/j.ccr.2012.05.026
M3 - Review article
SN - 0010-8545
VL - 257
SP - 332
EP - 349
JO - Coordination Chemistry Reviews
JF - Coordination Chemistry Reviews
IS - 2
ER -