Nitrite biosensing with disposable electrode strips: A preliminary study

Marcelo Rodrigues; Cátia Correia; Célia M. Silveira; José J. G. Moura; Estibaliz Ochoteco; Elena Jubete; M. Gabriela Almeida

doi:10.5220/0003757700320036

Nitrite biosensing with disposable electrode strips: A preliminary study

Marcelo Rodrigues, Cátia Correia, Célia M. Silveira, José J. G. Moura, Estibaliz Ochoteco, Elena Jubete, M. Gabriela Almeida

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM ^-1.cm ^-2 within a linear range of 0.001 - 1 mM.

Original language	English
Title of host publication	BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices
Editors	C. Correia, A. Fred, G. Gamboa, J. Gabriel
Publisher	SciTePress - Science and Technology Publications
Pages	32-36
Number of pages	5
ISBN (Print)	9789898425911
DOIs	https://doi.org/10.5220/0003757700320036
Publication status	Published - 13 Jun 2012
Event	International Conference on Biomedical Electronics and Devices, BIODEVICES 2012 - Vilamoura, Algarve, Portugal Duration: 1 Feb 2012 → 4 Feb 2012

Conference

Conference	International Conference on Biomedical Electronics and Devices, BIODEVICES 2012
Country/Territory	Portugal
City	Vilamoura, Algarve
Period	1/02/12 → 4/02/12

Keywords

Cytochrome c nitrite reductase
Electrochemical biosensors
Nitrite
Screen printed electrodes

Access to Document

10.5220/0003757700320036

Cite this

Rodrigues, M., Correia, C., Silveira, C. M., Moura, J. J. G., Ochoteco, E., Jubete, E., & Almeida, M. G. (2012). Nitrite biosensing with disposable electrode strips: A preliminary study. In C. Correia, A. Fred, G. Gamboa, & J. Gabriel (Eds.), BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices (pp. 32-36). SciTePress - Science and Technology Publications. https://doi.org/10.5220/0003757700320036

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title = "Nitrite biosensing with disposable electrode strips: A preliminary study",

abstract = "This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -1.cm -2 within a linear range of 0.001 - 1 mM.",

keywords = "Cytochrome c nitrite reductase, Electrochemical biosensors, Nitrite, Screen printed electrodes",

author = "Marcelo Rodrigues and C{\'a}tia Correia and Silveira, {C{\'e}lia M.} and Moura, {Jos{\'e} J. G.} and Estibaliz Ochoteco and Elena Jubete and Almeida, {M. Gabriela}",

year = "2012",

month = jun,

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doi = "10.5220/0003757700320036",

language = "English",

isbn = "9789898425911",

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editor = "C. Correia and A. Fred and G. Gamboa and J. Gabriel",

booktitle = "BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices",

publisher = "SciTePress - Science and Technology Publications",

address = "Portugal",

note = "International Conference on Biomedical Electronics and Devices, BIODEVICES 2012 ; Conference date: 01-02-2012 Through 04-02-2012",

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Rodrigues, M , Correia, C , Silveira, CM , Moura, JJG, Ochoteco, E, Jubete, E & Almeida, MG 2012, Nitrite biosensing with disposable electrode strips: A preliminary study. in C Correia, A Fred, G Gamboa & J Gabriel (eds), BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. SciTePress - Science and Technology Publications, pp. 32-36, International Conference on Biomedical Electronics and Devices, BIODEVICES 2012, Vilamoura, Algarve, Portugal, 1/02/12. https://doi.org/10.5220/0003757700320036

Nitrite biosensing with disposable electrode strips: A preliminary study. / Rodrigues, Marcelo ; Correia, Cátia ; Silveira, Célia M. et al.
BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. ed. / C. Correia; A. Fred; G. Gamboa; J. Gabriel. SciTePress - Science and Technology Publications, 2012. p. 32-36.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Nitrite biosensing with disposable electrode strips: A preliminary study

AU - Rodrigues, Marcelo

AU - Correia, Cátia

AU - Silveira, Célia M.

AU - Moura, José J. G.

AU - Ochoteco, Estibaliz

AU - Jubete, Elena

AU - Almeida, M. Gabriela

PY - 2012/6/13

Y1 - 2012/6/13

N2 - This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -1.cm -2 within a linear range of 0.001 - 1 mM.

AB - This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -1.cm -2 within a linear range of 0.001 - 1 mM.

KW - Cytochrome c nitrite reductase

KW - Electrochemical biosensors

KW - Nitrite

KW - Screen printed electrodes

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U2 - 10.5220/0003757700320036

DO - 10.5220/0003757700320036

M3 - Conference contribution

AN - SCOPUS:84861967204

SN - 9789898425911

SP - 32

EP - 36

BT - BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices

A2 - Correia, C.

A2 - Fred, A.

A2 - Gamboa, G.

A2 - Gabriel, J.

PB - SciTePress - Science and Technology Publications

T2 - International Conference on Biomedical Electronics and Devices, BIODEVICES 2012

Y2 - 1 February 2012 through 4 February 2012

ER -

Rodrigues M , Correia C , Silveira CM , Moura JJG, Ochoteco E, Jubete E et al. Nitrite biosensing with disposable electrode strips: A preliminary study. In Correia C, Fred A, Gamboa G, Gabriel J, editors, BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. SciTePress - Science and Technology Publications. 2012. p. 32-36 doi: 10.5220/0003757700320036

Nitrite biosensing with disposable electrode strips: A preliminary study

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