Nitrite biosensing with disposable electrode strips: A preliminary study

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This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -2 within a linear range of 0.001 - 1 mM.

Original languageEnglish
Title of host publicationBIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices
EditorsC. Correia, A. Fred, G. Gamboa, J. Gabriel
PublisherSciTePress - Science and Technology Publications
Number of pages5
ISBN (Print)9789898425911
Publication statusPublished - 13 Jun 2012
EventInternational Conference on Biomedical Electronics and Devices, BIODEVICES 2012 - Vilamoura, Algarve, Portugal
Duration: 1 Feb 20124 Feb 2012


ConferenceInternational Conference on Biomedical Electronics and Devices, BIODEVICES 2012
CityVilamoura, Algarve


  • Cytochrome c nitrite reductase
  • Electrochemical biosensors
  • Nitrite
  • Screen printed electrodes


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