Nitrite biosensing with disposable electrode strips: A preliminary study

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -1.cm -2 within a linear range of 0.001 - 1 mM.

Original languageEnglish
Title of host publicationBIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices
EditorsC. Correia, A. Fred, G. Gamboa, J. Gabriel
PublisherSCITEPRESS - Science and Technology Publications, Lda
Pages32-36
Number of pages5
ISBN (Print)9789898425911
DOIs
Publication statusPublished - 13 Jun 2012
EventInternational Conference on Biomedical Electronics and Devices, BIODEVICES 2012 - Vilamoura, Algarve, Portugal
Duration: 1 Feb 20124 Feb 2012

Conference

ConferenceInternational Conference on Biomedical Electronics and Devices, BIODEVICES 2012
CountryPortugal
CityVilamoura, Algarve
Period1/02/124/02/12

Fingerprint

Ink
Electrodes
Enzymes
Enzyme electrodes
Screen printing
Biocompatibility
Thick films
Biosensors
Organic solvents
Curing
Graphite
Heat treatment
Plastics
Carbon
Chemical analysis

Keywords

  • Cytochrome c nitrite reductase
  • Electrochemical biosensors
  • Nitrite
  • Screen printed electrodes

Cite this

Rodrigues, M., Correia, C., Silveira, C. M., Moura, J. J. G., Ochoteco, E., Jubete, E., & Almeida, M. G. (2012). Nitrite biosensing with disposable electrode strips: A preliminary study. In C. Correia, A. Fred, G. Gamboa, & J. Gabriel (Eds.), BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices (pp. 32-36). SCITEPRESS - Science and Technology Publications, Lda. https://doi.org/10.5220/0003757700320036
Rodrigues, Marcelo ; Correia, Cátia ; Silveira, Célia M. ; Moura, José J. G. ; Ochoteco, Estibaliz ; Jubete, Elena ; Almeida, M. Gabriela. / Nitrite biosensing with disposable electrode strips: A preliminary study. BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. editor / C. Correia ; A. Fred ; G. Gamboa ; J. Gabriel. SCITEPRESS - Science and Technology Publications, Lda, 2012. pp. 32-36
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abstract = "This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -1.cm -2 within a linear range of 0.001 - 1 mM.",
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author = "Marcelo Rodrigues and C{\'a}tia Correia and Silveira, {C{\'e}lia M.} and Moura, {Jos{\'e} J. G.} and Estibaliz Ochoteco and Elena Jubete and Almeida, {M. Gabriela}",
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Rodrigues, M, Correia, C, Silveira, CM, Moura, JJG, Ochoteco, E, Jubete, E & Almeida, MG 2012, Nitrite biosensing with disposable electrode strips: A preliminary study. in C Correia, A Fred, G Gamboa & J Gabriel (eds), BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. SCITEPRESS - Science and Technology Publications, Lda, pp. 32-36, International Conference on Biomedical Electronics and Devices, BIODEVICES 2012, Vilamoura, Algarve, Portugal, 1/02/12. https://doi.org/10.5220/0003757700320036

Nitrite biosensing with disposable electrode strips: A preliminary study. / Rodrigues, Marcelo; Correia, Cátia; Silveira, Célia M.; Moura, José J. G.; Ochoteco, Estibaliz; Jubete, Elena; Almeida, M. Gabriela.

BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. ed. / C. Correia; A. Fred; G. Gamboa; J. Gabriel. SCITEPRESS - Science and Technology Publications, Lda, 2012. p. 32-36.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Correia, Cátia

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N2 - This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -1.cm -2 within a linear range of 0.001 - 1 mM.

AB - This paper presents the results of a preliminary study on the construction of miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, now we aim to reduce the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with ccNiR previously mixed with the conductive graphite ink, in the presence of propanone or methylethylketone. Then, the enzyme electrodes were dried at 40°C or 60°C, to simulate the curing procedure typically done after screen-printing. In this way, the biocompatibility of ccNiR with these organic solvents and thermal treatments was evaluated and the composition of the mix enzyme/conductive ink was optimized. The analytical performance of these electrodes was satisfactory, with a sensitivity of 52 A.μM -1.cm -2 within a linear range of 0.001 - 1 mM.

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M3 - Conference contribution

SN - 9789898425911

SP - 32

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BT - BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices

A2 - Correia, C.

A2 - Fred, A.

A2 - Gamboa, G.

A2 - Gabriel, J.

PB - SCITEPRESS - Science and Technology Publications, Lda

ER -

Rodrigues M, Correia C, Silveira CM, Moura JJG, Ochoteco E, Jubete E et al. Nitrite biosensing with disposable electrode strips: A preliminary study. In Correia C, Fred A, Gamboa G, Gabriel J, editors, BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. SCITEPRESS - Science and Technology Publications, Lda. 2012. p. 32-36 https://doi.org/10.5220/0003757700320036