Nitrite Biosensing Using Cytochrome C Nitrite Reductase: Towards a Disposable Strip Electrode

Cátia Correia, Marcelo Rodrigues, Célia M. Silveira, José J.G. Moura, Estibaliz Ochoteco, Elena Jubete, M. Gabriela Almeida

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents the results of a primary study that aims to produce miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, here we aimed at reducing the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with the ccNiR enzyme, previously mixed with the conductive graphite ink. Firstly, though, the electrode preparation was optimized (enzyme amount, organic solvent and curing temperature). Then, the biocompatibility of ccNiR with these harsh treatments and the analytical performance of the modified electrodes were evaluated by cyclic voltammetry. Finally, the carbon paste screen-printed electrodes were coated with the ccNiR/carbon ink composite, displaying a good sensitivity (5.3x10- 7 A.uM- 1.cm- 2) within the linear range of 0.001 - 1.5 mM.

Original languageEnglish
Title of host publicationBiomedical Engineering Systems and Technologies - 5th International Joint Conference, BIOSTEC 2012, Revised Selected Papers
PublisherSpringer Verlag
Pages41-50
Number of pages10
ISBN (Print)9783642382550
DOIs
Publication statusPublished - 1 Jan 2013
Event5th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2012 - Vilamoura, Algarve, Portugal
Duration: 1 Feb 20124 Feb 2012

Publication series

NameCommunications in Computer and Information Science
Volume357 CCIS
ISSN (Print)1865-0929

Conference

Conference5th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2012
CountryPortugal
CityVilamoura, Algarve
Period1/02/124/02/12

Keywords

  • Cytochrome c nitrite reductase
  • Electrochemical biosensors
  • Nitrite
  • Screen printed electrodes

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