Nitric Oxide Detection Using Electrochemical Third-generation Biosensors – Based on Heme Proteins and Porphyrins

Filipa O. Gomes, Luísa B. Maia, Cristina Cordas, Cristina Delerue-Matos, Isabel Moura, José J. G. Moura, Simone Morais

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Nitric oxide radical (NO) is a signalling molecule involved in virtually all forms of life. Its relevance has been leading to the development of different analytical methodologies to assess the temporal and spatial fluxes of NO under the complex biological milieu. Third-generation electrochemical biosensors are promising tools for in loco and in vivo NO quantification and, over the past years, heme proteins and porphyrins have been used in their design. Since there are some limitations with the biorecognition element directly adsorbed onto the electrode surface, nanomaterials (carbon nanotubes, gold nanoparticles, etc.) and polymers (cellulose, chitosan, nafion®, polyacrylamide, among others) have been explored to achieve high kinetics and better biosensor performance. In this review, a broad overview of the field of electrochemical third-generation biosensors for NO electroanalysis is presented, discussing their main characteristics and aiming new outlooks and advances in this field.

Original languageEnglish
Pages (from-to)2485-2503
JournalElectroanalysis
Volume30
Issue number11
DOIs
Publication statusPublished - Nov 2018

Keywords

  • Heme proteins
  • Kinetic and electroanalytical parameters.
  • Nitric oxide detection
  • Porphyrins
  • Third-generation biosensors

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