TY - JOUR
T1 - Nitration of tyrosine 74 prevents human cytochrome c to play a key role in apoptosis signaling by blocking caspase-9 activation
AU - Teixeira, Miguel Nuno
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Tyrosine nitration is one of the most common post-transcriptional modifications of proteins, so affecting their structure and function. Human cytochrome c, with five tyrosine residues, is an excellent case study as it is a well-known protein playing a double physiological role in different cell compartments. On one hand, it acts as electron carrier within the mitochondrial respiratory electron transport chain, and on the other hand, it serves as a cytoplasmic apoptosis-triggering agent. In a previous paper, we reported the effect of nitration on physicochemical and kinetic features of monotyrosine cytochrome c mutants. Here, we analyse the nitration-induced changes in secondary structure, thermal stability, haem environment, alkaline transition and molecular dynamics of three of such monotyrosine mutants the so-called h-Y67, h-Y74 and h-Y97 which have four tyrosines replaced by phenylalanines and just keep the tyrosine residue giving its number to the mutant. The resulting data, along with the functional analyses of the three mutants, indicate that it is the specific nitration of solvent-exposed Tyr74 which enhances the peroxidase activity and blocks the ability of Cc to activate caspase-9, thereby preventing the apoptosis signaling pathway.
AB - Tyrosine nitration is one of the most common post-transcriptional modifications of proteins, so affecting their structure and function. Human cytochrome c, with five tyrosine residues, is an excellent case study as it is a well-known protein playing a double physiological role in different cell compartments. On one hand, it acts as electron carrier within the mitochondrial respiratory electron transport chain, and on the other hand, it serves as a cytoplasmic apoptosis-triggering agent. In a previous paper, we reported the effect of nitration on physicochemical and kinetic features of monotyrosine cytochrome c mutants. Here, we analyse the nitration-induced changes in secondary structure, thermal stability, haem environment, alkaline transition and molecular dynamics of three of such monotyrosine mutants the so-called h-Y67, h-Y74 and h-Y97 which have four tyrosines replaced by phenylalanines and just keep the tyrosine residue giving its number to the mutant. The resulting data, along with the functional analyses of the three mutants, indicate that it is the specific nitration of solvent-exposed Tyr74 which enhances the peroxidase activity and blocks the ability of Cc to activate caspase-9, thereby preventing the apoptosis signaling pathway.
KW - STRUCTURAL TRANSITIONS
KW - CARDIOLIPIN COMPLEX
KW - ALKALINE TRANSITION
KW - PROTEIN
KW - NUCLEAR-MAGNETIC-RESONANCE
KW - NITRIC-OXIDE
KW - FERRICYTOCHROME-C
KW - PEROXIDASE-ACTIVITY
KW - MOLECULAR-DYNAMICS
KW - OXIDATIVE STRESS
U2 - 10.1016/j.bbabio.2010.03.009
DO - 10.1016/j.bbabio.2010.03.009
M3 - Article
VL - 1797
SP - 981
EP - 993
JO - Biochimica Et Biophysica Acta-Bioenergetics
JF - Biochimica Et Biophysica Acta-Bioenergetics
SN - 0005-2728
IS - 6-7
ER -