New microsatellite markers for multi-scale genetic studies on Phlebotomus ariasi Tonnoir, vector of Leishmania infantum in the Mediterranean area

Jorian Prudhomme; Céline Toty; Ozge Erisoz Kasap; Nil Rahola; Baptiste Vergnes; Carla Maia; Lenea Campino; Maria Antoniou; Maribel Jimenez; Ricardo Molina; Arnaud Cannet; Bulent Alten; Denis Sereno; Anne Laure Bañuls

doi:10.1016/j.actatropica.2014.10.016

New microsatellite markers for multi-scale genetic studies on Phlebotomus ariasi Tonnoir, vector of Leishmania infantum in the Mediterranean area

Jorian Prudhomme, Céline Toty, Ozge Erisoz Kasap, Nil Rahola, Baptiste Vergnes, Carla Maia, Lenea Campino, Maria Antoniou, Maribel Jimenez, Ricardo Molina, Arnaud Cannet, Bulent Alten, Denis Sereno, Anne Laure Bañuls

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The population structure of Phlebotomus ariasi, a proven vector of Leishmania infantum in the Mediterranean area, is still poorly understood. Previously, only two microsatellite loci had been developed to study the population genetics of this species. Herein we use these loci and determined fourteen novel microsatellite loci, useful for the characterization of P. ariasi populations. These loci were tested on three populations of P. ariasi, two from France and one from Portugal. In addition, the usefulness of these markers was also evaluated on seven other sandfly species.We show, that for P. ariasi, 15 of the 16 loci selected were polymorphic, with a mean of 4.25 alleles and an observed heterozygosity of 0.299. Within the P. ariasi population of France, 11 loci were polymorphic, with an average of 2.44 alleles and an observed heterozygosity of 0.2177. The fixation index was moderate among the French populations but high between French and Portuguese populations. In addition, eight loci were also found to be amplifiable in six other Phlebotomus species.These results demonstrate the usefulness of this new set of microsatellite loci for population structure and molecular ecology studies of P. ariasi at various spatial scales, but also of other sandfly species.

Original language	English
Pages (from-to)	79-85
Number of pages	7
Journal	Acta Tropica
Volume	142
DOIs	https://doi.org/10.1016/j.actatropica.2014.10.016
Publication status	Published - 1 Feb 2015

Keywords

Microsatellites
Phlebotomus ariasi
Population genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.actatropica.2014.10.016

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Prudhomme, J., Toty, C., Kasap, O. E., Rahola, N., Vergnes, B., Maia, C., Campino, L., Antoniou, M., Jimenez, M., Molina, R., Cannet, A., Alten, B., Sereno, D., & Bañuls, A. L. (2015). New microsatellite markers for multi-scale genetic studies on Phlebotomus ariasi Tonnoir, vector of Leishmania infantum in the Mediterranean area. Acta Tropica, 142, 79-85. https://doi.org/10.1016/j.actatropica.2014.10.016

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title = "New microsatellite markers for multi-scale genetic studies on Phlebotomus ariasi Tonnoir, vector of Leishmania infantum in the Mediterranean area",

abstract = "The population structure of Phlebotomus ariasi, a proven vector of Leishmania infantum in the Mediterranean area, is still poorly understood. Previously, only two microsatellite loci had been developed to study the population genetics of this species. Herein we use these loci and determined fourteen novel microsatellite loci, useful for the characterization of P. ariasi populations. These loci were tested on three populations of P. ariasi, two from France and one from Portugal. In addition, the usefulness of these markers was also evaluated on seven other sandfly species.We show, that for P. ariasi, 15 of the 16 loci selected were polymorphic, with a mean of 4.25 alleles and an observed heterozygosity of 0.299. Within the P. ariasi population of France, 11 loci were polymorphic, with an average of 2.44 alleles and an observed heterozygosity of 0.2177. The fixation index was moderate among the French populations but high between French and Portuguese populations. In addition, eight loci were also found to be amplifiable in six other Phlebotomus species.These results demonstrate the usefulness of this new set of microsatellite loci for population structure and molecular ecology studies of P. ariasi at various spatial scales, but also of other sandfly species.",

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Prudhomme, J, Toty, C, Kasap, OE, Rahola, N, Vergnes, B, Maia, C , Campino, L, Antoniou, M, Jimenez, M, Molina, R, Cannet, A, Alten, B, Sereno, D & Bañuls, AL 2015, 'New microsatellite markers for multi-scale genetic studies on Phlebotomus ariasi Tonnoir, vector of Leishmania infantum in the Mediterranean area', Acta Tropica, vol. 142, pp. 79-85. https://doi.org/10.1016/j.actatropica.2014.10.016

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AU - Prudhomme, Jorian

AU - Toty, Céline

AU - Kasap, Ozge Erisoz

AU - Rahola, Nil

AU - Vergnes, Baptiste

AU - Maia, Carla

AU - Campino, Lenea

AU - Antoniou, Maria

AU - Jimenez, Maribel

AU - Molina, Ricardo

AU - Cannet, Arnaud

AU - Alten, Bulent

AU - Sereno, Denis

AU - Bañuls, Anne Laure

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N2 - The population structure of Phlebotomus ariasi, a proven vector of Leishmania infantum in the Mediterranean area, is still poorly understood. Previously, only two microsatellite loci had been developed to study the population genetics of this species. Herein we use these loci and determined fourteen novel microsatellite loci, useful for the characterization of P. ariasi populations. These loci were tested on three populations of P. ariasi, two from France and one from Portugal. In addition, the usefulness of these markers was also evaluated on seven other sandfly species.We show, that for P. ariasi, 15 of the 16 loci selected were polymorphic, with a mean of 4.25 alleles and an observed heterozygosity of 0.299. Within the P. ariasi population of France, 11 loci were polymorphic, with an average of 2.44 alleles and an observed heterozygosity of 0.2177. The fixation index was moderate among the French populations but high between French and Portuguese populations. In addition, eight loci were also found to be amplifiable in six other Phlebotomus species.These results demonstrate the usefulness of this new set of microsatellite loci for population structure and molecular ecology studies of P. ariasi at various spatial scales, but also of other sandfly species.

AB - The population structure of Phlebotomus ariasi, a proven vector of Leishmania infantum in the Mediterranean area, is still poorly understood. Previously, only two microsatellite loci had been developed to study the population genetics of this species. Herein we use these loci and determined fourteen novel microsatellite loci, useful for the characterization of P. ariasi populations. These loci were tested on three populations of P. ariasi, two from France and one from Portugal. In addition, the usefulness of these markers was also evaluated on seven other sandfly species.We show, that for P. ariasi, 15 of the 16 loci selected were polymorphic, with a mean of 4.25 alleles and an observed heterozygosity of 0.299. Within the P. ariasi population of France, 11 loci were polymorphic, with an average of 2.44 alleles and an observed heterozygosity of 0.2177. The fixation index was moderate among the French populations but high between French and Portuguese populations. In addition, eight loci were also found to be amplifiable in six other Phlebotomus species.These results demonstrate the usefulness of this new set of microsatellite loci for population structure and molecular ecology studies of P. ariasi at various spatial scales, but also of other sandfly species.

KW - Microsatellites

KW - Phlebotomus ariasi

KW - Population genetics

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AN - SCOPUS:84911926520

SN - 0001-706X

VL - 142

SP - 79

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JO - Acta Tropica

JF - Acta Tropica

ER -

New microsatellite markers for multi-scale genetic studies on Phlebotomus ariasi Tonnoir, vector of Leishmania infantum in the Mediterranean area

Abstract

Keywords

UN SDGs

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