New methods for the identification of efflux mediated MDR bacteria, genetic assessment of regulators and efflux pump constituents, characterization of efflux systems and screening for inhibitors of efflux pumps

M. Viveiros, M. Martins, I. Couto, Liliana Rodrigues, Gabriella Spengler, A. Martins, Jette E. Kristiansen, Joseph Molnar, L. Amaral

Research output: Contribution to journalReview article

41 Citations (Scopus)

Abstract

We have developed a number of methods that identify efflux pump mediated multi-drug resistant bacteria, characterize efflux systems and screen for inhibitors of efflux pumps. These approaches were complemented by the quantification of the expression of genes that regulate and code for constituents of efflux pumps. The methods described are easy to use, reproducible and for the most part, require instrumentation normally present in a clinical bacteriology laboratory. Because each method provides good reproducibility, they lend themselves for inter-laboratory use.

Original languageEnglish
Pages (from-to)760-778
Number of pages19
JournalCurrent Drug Targets
VolumeVol. 9
Issue numbern.º 9
DOIs
Publication statusPublished - Sep 2008

Keywords

  • EB based methods
  • Efflux pump inhibitors
  • Fluorometric assay
  • Intrinsic efflux pumps
  • MDR bacteria
  • MDR efflux pumps

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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