New branches in the degradation pathway of monochlorocatechols by Aspergillus nidulans: A metabolomics analysis

Tiago M. Martins, Oscar Núñez, Hector Gallart-Ayala, Maria Cristina Leitão, Maria Teresa Galceran, Cristina Maria Pereira

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A collective view of the degradation of monochlorocatechols in fungi is yet to be attained, though these compounds are recognised as key degradation intermediates of numerous chlorinated aromatic hydrocarbons, including monochlorophenols. In the present contribution we have analysed the degradation pathways of monochlorophenols in Aspergillus nidulans using essentially metabolomics. Degradation intermediates herein identified included those commonly reported (e.g. 3-chloro-. cis,. cis-muconate) but also compounds never reported before in fungi revealing for 4-chlorocatechol and for 3-chlorocatechol unknown degradation paths yielding 3-chlorodienelactone and catechol, respectively. A different 3-chlorocatechol degradation path led to accumulation of 2-chloromuconates (a potential dead-end), notwithstanding preliminary evidence of chloromuconolactones and protoanemonin simultaneous formation. In addition, some transformation intermediates, of which sulfate conjugates of mono-chlorophenols/chlorocatechols were the most common, were also identified. This study provides critical information for understanding the role of fungi in the degradation of chlorinated aromatic hydrocarbons; furthering their utility in the development of innovative bioremediation strategies.

Original languageEnglish
Pages (from-to)264-272
Number of pages9
JournalJournal of Hazardous Materials
Volume268
DOIs
Publication statusPublished - 15 Mar 2014

Keywords

  • Aspergillus nidulans
  • Biodegradation
  • Chlorinated aromatic hydrocarbons
  • Metabolic pathway
  • Monochlorocatechols
  • Monochlorophenols

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