Neuroprotective effect of blackberry (Rubus sp.) polyphenols is potentiated after simulated gastrointestinal digestion

Research output: Contribution to journalArticlepeer-review

91 Citations (Scopus)

Abstract

Blackberry ingestion has been demonstrated to attenuate brain degenerative processes in rodents with the benefits ascribed to the (poly)phenolic components. The aim of this work was to assess the efficacy of blackberry polyphenolics in a neurodegeneration cell model before and after simulated gastrointestinal digestion. Digested blackberry metabolites protected neuroblastoma cells from H(2)O(2)-induced death at low, nontoxic levels that approach physiologically-relevant serum concentrations. However, the original extracts were not protective even at fivefold higher concentrations. This potentiation may reflect alterations in the polyphenolic composition caused by the digestion procedure, as detected by liquid-chromatographymass spectrometric analysis. This protection was not caused by modulation of the intracellular antioxidant capacity or through alteration of glutathione levels, although the original extract influenced both of these parameters. This work reinforces the importance of evaluating digested metabolites in disease cell models and highlights the possible involvement of other mechanisms beyond antioxidant systems. (C) 2011 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1443-1452
JournalFood Chemistry
Volume131
Issue number4
DOIs
Publication statusPublished - 15 Apr 2012

Fingerprint

Dive into the research topics of 'Neuroprotective effect of blackberry (Rubus sp.) polyphenols is potentiated after simulated gastrointestinal digestion'. Together they form a unique fingerprint.

Cite this