Nested and multiplex real-time PCR using dual-labeled probes: Detecting and discriminating Mycobacterium tuberculosis complex members in cultures and animal tissues

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Abstract

Members of the Mycobacterium tuberculosis complex (MTC) are causative agents of tuberculosis (TB) in both humans and animals. In the last two decades, the accumulating knowledge of the nucleotide sequences of several genes, and of the whole genomes, of MTC members has allowed the development of novel molecular assays able to detect and discriminate between these species. However, despite the significant advances in the development of molecular assays for detecting MTC members in human samples, only a few assays have been described for detecting these agents in animal tissues. In this chapter we describe the use of two TaqMan ® -based real-time PCR approaches, highly sensitive and specific and easy to perform, to detect and identify veterinary-relevant MTC species in both animal tissue samples and cultures.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationVeterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies
Editors M, Cunha , J Inácio
PublisherSpringer New York
Pages133-143
Number of pages11
Volume1247
ISBN (Electronic)9781493920044
ISBN (Print)9781493920037
DOIs
Publication statusPublished - 15 Nov 2014

Keywords

  • Bovine tuberculosis
  • Mycobacterium bovis
  • Mycobacterium tuberculosis complex
  • Real-time PCR

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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    Costa, P., Couto, I., Viveiros, M., & Inácio, J. (2014). Nested and multiplex real-time PCR using dual-labeled probes: Detecting and discriminating Mycobacterium tuberculosis complex members in cultures and animal tissues. In M. Cunha , & J. Inácio (Eds.), Methods in Molecular Biology: Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies (Vol. 1247, pp. 133-143). Springer New York. https://doi.org/10.1007/978-1-4939-2004-4_9