Neoglycolipid-based oligosaccharide microarray system: Preparation of ngls and their noncovalent immobilization on nitrocellulose-coated glass slides for microarray analyses

Yan Liu, Robert A. Childs, Angelina S. Palma, Maria A. Campanero-Rhodes, Mark S. Stoll, Wengang Chai, Ten Feizi

Research output: Chapter in Book/Report/Conference proceedingChapter

47 Citations (Scopus)

Abstract

Carbohydrate microarrays, since their advent in 2002, are revolutionizing studies of the molecular basis of protein-carbohydrate interactions both in endogenous recognition systems and pathogen-host interactions. We have developed a unique carbohydrate microarray system based on the neoglycolipid (NGL) technology, a well-validated microscale approach for generating lipid-tagged oligosaccharide probes for use in carbohydrate recognition studies. This chapter provides an overview of the principles and key features of the NGL-based oligosaccharide microarrays, and describes in detail the basic techniques - from the preparation of NGL probes to the generation of microarrays using robotic arraying hardware, as well as a general protocol for probing the microarrays with carbohydrate-binding proteins.

Original languageEnglish
Title of host publicationCarbohydrate Microarrays: Methods and Protocols
EditorsYann Chevolot
PublisherHumana Press
Pages117-136
Number of pages20
ISBN (Electronic)978-1-61779-373-8
ISBN (Print)978-1-61779-372-1
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume808
ISSN (Print)1064-3745

Keywords

  • Microarrays
  • Neoglycolipids
  • Nitrocellulose
  • Noncovalent immobilization
  • Reductive amination

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