Negeviruses found in multiple species of mosquitoes from southern Portugal: Isolation, genetic diversity, and replication in insect cell culture

Sara Carapeta, Beatriz do Bem, James McGuinness, Aida Esteves, Ana Abecasis, Ângela Lopes, António P. de Matos, João Piedade, António Paulo Gouveia de Almeida, Ricardo Parreira

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In this report, an RT-PCR approach based on the use of degenerate primers allowed the identification of negeviruses in four different species of mosquitoes (Ochlerotatus caspius, Culex pipiens, Cx. theileri and Cx. univittatus) collected in southern Portugal. The genomes of two of these viruses, sequenced to full completion, were shown to encode all the proteins encoded by previously described negeviruses. One of these viruses induces exuberant cytopathic effect in insect cell culture, with no obvious signs of apoptosis induction, replicating very rapidly and allowing for the detection of viral genomes in the infected culture supernatant as soon as 4. h post-infection. This virus was also shown to use a dsRNA intermediate, which was found to be fully formed and active 3 h after infection. Phylogenetic analysis of two products encoded by the viral ORF1 placed both viruses among Negev virus cluster, in the recently proposed Nelorpivirus taxon.

Original languageEnglish
Pages (from-to)318-328
Number of pages11
JournalVirology
Volume483
DOIs
Publication statusPublished - 1 Sep 2015

Keywords

  • Cytopathic effect
  • dsRNA intermediate
  • Mosquitoes
  • Negevirus
  • Phylogenetic analysis
  • Portugal
  • RT-PCR
  • Viral replication

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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