TY - JOUR
T1 - Natural history of drug-resistant clones of Streptococcus pneumoniae colonizing healthy children in Portugal
AU - Mato, Rosario
AU - Santos Sanches, Ilda
AU - Simas, Carla
AU - Nunes, Sónia
AU - Carriço, João André
AU - Sousa, Natacha G.
AU - Frazão, Nelson
AU - Saldanha, Joana
AU - Brito-Avô, António
AU - Almeida, Jonas S.
AU - De Lencastre, Hermínia
PY - 2005/12
Y1 - 2005/12
N2 - A total of 3,539 Streptococcus pneumoniae (Pn) were recovered from 4,969 nasopharyngeal samples of children attending 13 day-care centers (DCCs) located in Lisbon, Portugal, during a surveillance study from January, 2001, through March, 2003, integrated in the European intervention project (EURIS, European Resistance Intervention Study). All Pn isolates were tested for antibiotyping and drug-resistant pneumococci (DRPn) were further tested by serotyping and pulsed-field gel electrophoresis (PFGE). Overall carriage of Pn was very high (71.2%) and 39.9% of the isolates were resistant to antimicrobials (22.5% with decreased susceptibility to penicillin and 17.4% susceptible to penicillin and resistant to other antimicrobials). Serotypes 6B, 14, 23F, 19F, and 19A were prevalent among the 1,287 DRPn and 5.8% of the isolates were nontypeable. Eighty PFGE patterns were identified among 1,285 DRPn, and 93.1% of the DRPn belonged to 26 major clonal types that comprised: Pneumococcal Molecular Epidemiology Network (PMEN) clones (76.3%), Portuguese (PT)-DCC clones, previously detected in 1996-1999 (14.3%), and EURIS PT-DCC new clones, identified for the first time in the EURIS study, during 2001-2003 (9.4%). Comparing with previous Portuguese surveillance studies carried out since 1996, we observed that carriage increased from 47% to 71%, but no major changes were detected on the prevalence of pneumococcal serotypes. Moreover, although PMEN clones were predominant in all DCCs, in the present study the majority of them, were gradually decreasing in time whereas several PT-DCC and new clones seemed to be increasing.
AB - A total of 3,539 Streptococcus pneumoniae (Pn) were recovered from 4,969 nasopharyngeal samples of children attending 13 day-care centers (DCCs) located in Lisbon, Portugal, during a surveillance study from January, 2001, through March, 2003, integrated in the European intervention project (EURIS, European Resistance Intervention Study). All Pn isolates were tested for antibiotyping and drug-resistant pneumococci (DRPn) were further tested by serotyping and pulsed-field gel electrophoresis (PFGE). Overall carriage of Pn was very high (71.2%) and 39.9% of the isolates were resistant to antimicrobials (22.5% with decreased susceptibility to penicillin and 17.4% susceptible to penicillin and resistant to other antimicrobials). Serotypes 6B, 14, 23F, 19F, and 19A were prevalent among the 1,287 DRPn and 5.8% of the isolates were nontypeable. Eighty PFGE patterns were identified among 1,285 DRPn, and 93.1% of the DRPn belonged to 26 major clonal types that comprised: Pneumococcal Molecular Epidemiology Network (PMEN) clones (76.3%), Portuguese (PT)-DCC clones, previously detected in 1996-1999 (14.3%), and EURIS PT-DCC new clones, identified for the first time in the EURIS study, during 2001-2003 (9.4%). Comparing with previous Portuguese surveillance studies carried out since 1996, we observed that carriage increased from 47% to 71%, but no major changes were detected on the prevalence of pneumococcal serotypes. Moreover, although PMEN clones were predominant in all DCCs, in the present study the majority of them, were gradually decreasing in time whereas several PT-DCC and new clones seemed to be increasing.
KW - CARRIAGE
KW - RESPIRATORY-TRACT INFECTIONS
KW - DAY-CARE-CENTERS
KW - NASOPHARYNX
KW - FIELD GEL-ELECTROPHORESIS
KW - LISBON
KW - PATTERNS
KW - MOLECULAR EPIDEMIOLOGY
KW - DISEASE
KW - HAEMOPHILUS-INFLUENZAE
UR - http://www.scopus.com/inward/record.url?scp=30744449963&partnerID=8YFLogxK
U2 - 10.1089/mdr.2005.11.309
DO - 10.1089/mdr.2005.11.309
M3 - Article
C2 - 16359190
SN - 1076-6294
VL - 11
SP - 309
EP - 322
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
IS - 4
ER -